FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184888
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT27440612.559270772689493TruSeq Adapter, Index 22 (97% over 38bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT399611.8289724690693527TruSeq Adapter, Index 22 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATG370461.695556019347445TruSeq Adapter, Index 22 (97% over 37bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTC268161.2273397995686735TruSeq Adapter, Index 22 (96% over 32bp)
AGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTC239201.094792959639121TruSeq Adapter, Index 22 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAAGCTCGTAT201440.9219694556425776TruSeq Adapter, Index 22 (97% over 38bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCT183480.839768445796764TruSeq Adapter, Index 22 (96% over 31bp)
CACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTG163710.7492832584553533TruSeq Adapter, Index 22 (96% over 27bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT151910.6952759134564335Illumina Single End PCR Primer 1 (96% over 25bp)
GAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTT131240.6006715218354443TruSeq Adapter, Index 22 (96% over 30bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCG129220.5914261966746122TruSeq Adapter, Index 22 (97% over 34bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACCGAACTTAATCTCGTAT115020.5264343069301493TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCC110590.5061586680873345TruSeq Adapter, Index 22 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT100640.46061857632977066TruSeq Adapter, Index 2 (97% over 36bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT80650.3691264723866853TruSeq Adapter, Index 2 (97% over 36bp)
GCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCT67170.3074299460658853TruSeq Adapter, Index 22 (96% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATG57520.2632629224015144TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTAT53340.24413150696969366TruSeq Adapter, Index 22 (97% over 38bp)
AAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATGCCGTC52750.24143113971974764TruSeq Adapter, Index 22 (96% over 32bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGC51300.23479464393598207TruSeq Adapter, Index 22 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT50310.23026351922844557Illumina Single End PCR Primer 1 (96% over 25bp)
AGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTATGCCGTCT40410.1849522721530806TruSeq Adapter, Index 22 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAACCTCGTAT32500.14874904342922843TruSeq Adapter, Index 22 (97% over 38bp)
GTTCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT31410.1437602293572943TruSeq Adapter, Index 2 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT30850.1411971689166676TruSeq Adapter, Index 2 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATATCGTATGCCGT30680.14041909699719163TruSeq Adapter, Index 22 (96% over 33bp)
ACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGC29580.13538451398881773TruSeq Adapter, Index 22 (96% over 26bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTAT27800.12723764330254TruSeq Adapter, Index 2 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT27120.12412535562463614Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTACTCTCGTAT26300.1203723028365756TruSeq Adapter, Index 22 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACGCTAACGAATCTCGTAT25860.11835846963322603Illumina Single End PCR Primer 1 (96% over 25bp)
CACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGTCTTCTGCT24420.11176774278590024Illumina PCR Primer Index 12 (96% over 25bp)
AAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAATCTCGTATGCCGT23290.10659585296820706TruSeq Adapter, Index 2 (96% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAACTTAAACTCGTAT22860.10462778870129728TruSeq Adapter, Index 22 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)