FastQCFastQC Report
Sun 2 Apr 2023
EGAF00002238360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20252385
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACAAGATCTCGTAT995030.49131497352040265TruSeq Adapter, Index 14 (97% over 38bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA497920.24585746320741975No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC349290.17246857592328016No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC228740.11294472231295227No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)