FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2391914
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT642492.6860915568034636TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA120450.5035716167052829TruSeq Adapter, Index 10 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT112050.46845329723393064Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA51850.21677200768923965No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT39660.16580863693259876Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39330.16442898866765276No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC25680.10736171952670538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)