FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2402913
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTAT651062.709461391236387TruSeq Adapter, Index 10 (97% over 38bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTGAAATCTCGTA125230.5211591097971504TruSeq Adapter, Index 10 (97% over 38bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT120570.5017659815399059Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA50140.20866340146314077No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT41220.17154179115099047Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC39010.16234462088306983No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC26540.11044927552516468No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT24110.1003365498459578Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)