FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002238434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424969
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT481823.381266539833498TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA67420.4731330997376083TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC50640.35537615204260586Illumina Single End Adapter 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA27920.1959340869871555No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC23560.1653369301367258Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAACAGAAGAGCACACGTCTGAAC22510.15796834878513147Illumina Single End Adapter 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC21230.14898569723271174No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT21070.14786286578865926Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT17830.1251255290465968Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAACGGAAGAGCACACGTCTGAAC17690.12414305153305089Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAAAAAAA14420.1011951838952286Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAGAACGGAAGAGCACACGTCTGAAC14390.10098465299946877Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)