FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002238438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1405328
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT408152.9043041909077454TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA56280.40047590313435727TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC43590.3101766989628044Illumina Single End Adapter 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA24040.17106326779228764No Hit
GATCGGAAGAGCACGCGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT21030.1496447804355994TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAACAGAAGAGCACACGTCTGAAC18700.13306502111962473Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC18610.13242460123188324Illumina Single End Adapter 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC17310.12317409174228365No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT17150.12203556749740985Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT15740.11200232258945955Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAACGGAAGAGCACACGTCTGAAC14190.10097286896724467Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)