FastQCFastQC Report
Sat 10 Sep 2022
EGAF00002238440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1438128
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT485233.374039028514847TruSeq Adapter, Index 7 (97% over 39bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA68240.47450574635915577TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC44320.3081784097104013Illumina Single End Adapter 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA28460.19789615388894452No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAAAGGAAGAGCACACGTCTGAAC24760.172168263186587Illumina Single End Adapter 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT21830.1517945551439093Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC21420.14894362671472916No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAACGGAAGAGCACACGTCTGAAC19590.1362187510430226Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAACAGAAGAGCACACGTCTGAAC19490.1355234026456616Illumina Single End Adapter 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATGCCTAAATCTCGTAT17060.11862643658978894Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAGAACGGAAGAGCACACGTCTGAAC17000.11820922755137234Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)