FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402305
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT1685432.632536250615989TruSeq Adapter, Index 7 (97% over 39bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT341900.534026417048235Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGCACACGTCTGAAC296130.4625365395744189Illumina Single End Adapter 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA235470.36778941334410026TruSeq Adapter, Index 7 (97% over 39bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCACACGTCTGAAC177890.27785305448584535Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC128810.2011931640245193Illumina Single End Adapter 1 (96% over 25bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT118970.185823699433251Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA104930.16389409751644135No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT98580.15397579465520622Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC80870.1263138822658402No Hit
GATCTCGTATGCCGTCTTCTGCTTGAAAACAAAAGAGCACACGTCTGAAC66080.10321282725518387Illumina Single End Adapter 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)