FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6402305
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2057823.2141861407727372Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATCAGATCGGAAGAGCGTCGTGTAGGGA923251.44205875852525Illumina PCR Primer Index 9 (96% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC324240.5064426015317921Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT105080.1641283881352107Illumina Single End PCR Primer 1 (100% over 31bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66050.10316596913143002No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)