FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6434815
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTAT1716002.6667433329474117TruSeq Adapter, Index 7 (97% over 39bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT363610.5650667501707509Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAGCACACGTCTGAAC285380.4434937134944828Illumina Single End Adapter 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGCACACGTCTGAAC252860.39295613005191293Illumina Single End Adapter 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCTGATCTCGTA245180.38102105499536504TruSeq Adapter, Index 7 (97% over 39bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT120730.18762000150742483Illumina Single End PCR Primer 1 (96% over 25bp)
GATCTCGTATGCCGTCTTCTGCTTGAAAAAAGAAGAGCACACGTCTGAAC117930.1832686720597251Illumina Single End Adapter 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA103640.1610613514141432No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT102080.15863703929328193Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC82460.12814665223475735No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT72590.11280821593161576Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)