FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002238494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782031
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTAT199250.7162033780356869TruSeq Adapter, Index 9 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT123840.4451424157387175Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA44450.15977535836228998No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT42670.15337715503529614Illumina Single End PCR Primer 1 (96% over 25bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC36920.13270880159135537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)