FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002238502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7708867
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTAT6071057.875411522860623TruSeq Adapter, Index 6 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGT853141.106699596711164TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATG848161.1002395034185959TruSeq Adapter, Index 6 (97% over 39bp)
AAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTC690000.8950731670425758TruSeq Adapter, Index 6 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTTC528630.6857427946285751TruSeq Adapter, Index 6 (96% over 31bp)
AGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCT462470.5999195471915653TruSeq Adapter, Index 6 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTT311670.4043006579306661TruSeq Adapter, Index 6 (96% over 32bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA263060.34124340191626085No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCG258250.33500383389673216TruSeq Adapter, Index 6 (97% over 36bp)
CACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTTCTG250140.3244834811652607TruSeq Adapter, Index 6 (96% over 29bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC207980.2697932134514709No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC180850.2345999742893476No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCC178710.2318239502640271TruSeq Adapter, Index 6 (97% over 37bp)
CACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTTCTGCT124220.161139114217433TruSeq Adapter, Index 6 (96% over 27bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT119040.15441957942717136No Hit
GTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAAC110820.14375653387196846No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCG105580.13695916663239877No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC104330.13533765727181438No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGC102760.13330104151492042No Hit
GTCCCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGC102030.13235408004833915No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGC100650.13056393371425398TruSeq Adapter, Index 6 (97% over 38bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC92030.11938200516366414No Hit
GCACACGTCTGAACTCCAGTCACGCCAAGACATCTCGTATGCCGTCTTCT87850.11395967786186997TruSeq Adapter, Index 6 (96% over 30bp)
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT85320.11067774291604719No Hit
CCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCG84150.10916001015454022No Hit
CTCGTTCATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT80550.1044900631960572No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG77110.100027669435729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)