FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238508

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238508
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11186929
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT2049571.8321113864224934TruSeq Adapter, Index 27 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA945770.8454241552797913No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC627140.5606006795966972No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC464230.4149753699160869No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT400060.35761378301408725Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC329150.2942273076015768No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTA296710.26522917951834685TruSeq Adapter, Index 27 (97% over 39bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC205750.18392000163762548No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC196980.1760804953709816No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC177450.15862262109645997No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT173500.15509171462516658No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT172440.15414418023033846No Hit
CCTCTCCTTACTTCATCCCCATCCCATGCCAAAGGAAGACCCTCCCTCCT155990.13943951910305322No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCCACACGTCTGAAC152580.13639131883289865Illumina Single End PCR Primer 1 (97% over 43bp)
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT131680.1177088010480803No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG125150.11187163161579018No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG121820.10889494337543396No Hit
CGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTC120690.10788483595453228No Hit
GTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG120300.10753621480926534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)