FastQCFastQC Report
Fri 10 May 2024
EGAF00002238509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11186929
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT408100.3648007420088212Illumina Single End PCR Primer 1 (100% over 31bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC315160.28172164139059075Illumina Single End PCR Primer 1 (100% over 50bp)
GCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCG134340.12008657603887536No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA115820.10353154114055789No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT113910.10182419142912233No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)