FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11092780
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT1712751.544022328036795TruSeq Adapter, Index 27 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA809060.7293572936630853No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC528000.4759852805157949No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC385410.3474422101583192No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT337240.30401756818398995Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC287000.25872684755309305No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTA252850.22794105715609614TruSeq Adapter, Index 27 (97% over 39bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC176130.1587789535175132No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC169360.15267588467453605No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT154510.13928879866002933No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT146410.13198675174302563No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC144390.13016574745014325No Hit
CCTCTCCTTACTTCATCCCCATCCCATGCCAAAGGAAGACCCTCCCTCCT132850.11976258431159728No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCCACACGTCTGAAC126450.11399306575989067Illumina Single End PCR Primer 1 (97% over 43bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)