FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11327138
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTAT2023751.786638425346279TruSeq Adapter, Index 27 (97% over 39bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA961120.8485108948085563No Hit
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC626870.5534231153535871No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC464280.40988288480285134No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT401130.3541318204121818Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC340260.3003936210541445No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGCCTAAATCTCGTA299060.26402079678026347TruSeq Adapter, Index 27 (97% over 39bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC204870.1808665172084952No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC201830.17818269716498555No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT177600.15679159201556475No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC174720.15424902565855558No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT174480.15403714512880481No Hit
CCTCTCCTTACTTCATCCCCATCCCATGCCAAAGGAAGACCCTCCCTCCT160920.1420658951978867No Hit
AATGATACGGCGACCACCGAGATCTACACTCTTTCCCCACACGTCTGAAC154020.13597432996755227Illumina Single End PCR Primer 1 (97% over 43bp)
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGAT129890.11467150837219428No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG126630.1117934645097464No Hit
CGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTC124810.11018670382580313No Hit
GTATAATGCTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG123920.10940098019464405No Hit
CTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCG123530.10905667433379906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)