FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002238522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645977
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT1016232.7872638801616136TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGT299370.8210967869517553TruSeq Adapter, Index 1 (96% over 31bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA225600.6187641885837458No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG220320.6042824735317859TruSeq Adapter, Index 1 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTC188610.5173099007481397TruSeq Adapter, Index 1 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTC148180.40642055613625644TruSeq Adapter, Index 1 (96% over 27bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC146730.40244356999509323No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCG132120.3623720061865448TruSeq Adapter, Index 1 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCT115530.3168697992335114TruSeq Adapter, Index 1 (96% over 29bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCC108870.29860309047478906TruSeq Adapter, Index 1 (96% over 33bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC106710.2926787524989872No Hit
GAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTT95130.2609177183509386TruSeq Adapter, Index 1 (96% over 28bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC73470.2015097736491481No Hit
CACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTG67220.184367592006203TruSeq Adapter, Index 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA58480.1603959651967086TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT51640.14163556160666949Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC50160.1375762929936201No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT43220.11854161449729388No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT41250.11313839884343757No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC41170.11291897891840788No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGC39170.10743348079266546TruSeq Adapter, Index 1 (97% over 34bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC36960.10137200536372007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)