FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002238524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3642755
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT1019232.7979647272462738TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGT301700.8282193010509902TruSeq Adapter, Index 1 (96% over 31bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA228530.6273548454397839No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG218630.6001776128232615TruSeq Adapter, Index 1 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTC191800.5265245672574741TruSeq Adapter, Index 1 (96% over 30bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC147340.40447408623418263No Hit
AGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTC142270.390556048924509TruSeq Adapter, Index 1 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCG131130.35997479929339193TruSeq Adapter, Index 1 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCT112210.3080360880707047TruSeq Adapter, Index 1 (96% over 29bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC109190.2997456595351595No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCC109020.29927897978315865TruSeq Adapter, Index 1 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTT94950.2606543673675556TruSeq Adapter, Index 1 (96% over 28bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC71140.19529175033731339No Hit
CACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTG66360.1821698137810531TruSeq Adapter, Index 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA56340.1546631601631183TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT51660.141815741108035Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC48890.13421160632543225No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT43640.11979943751364008No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC42540.11677974500069316No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT40760.11189333347974267No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGC38450.1055519792025541TruSeq Adapter, Index 1 (97% over 34bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC37060.1017361859361939No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)