FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002238528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3698555
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT1026132.774407843063034TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGT303750.8212666838805966TruSeq Adapter, Index 1 (96% over 31bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA231030.6246493563026642No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG220310.5956650637884255TruSeq Adapter, Index 1 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTC195670.5290444511437574TruSeq Adapter, Index 1 (96% over 30bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC147730.3994262624187014No Hit
AGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTC147420.39858809724338284TruSeq Adapter, Index 1 (96% over 27bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCG134120.3626281074635905TruSeq Adapter, Index 1 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCT117920.31882721765662536TruSeq Adapter, Index 1 (96% over 29bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC111290.30090129793933035No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCC110490.29873829103528265TruSeq Adapter, Index 1 (96% over 33bp)
GAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTT97120.2625890381513861TruSeq Adapter, Index 1 (96% over 28bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC72790.19680659068203663No Hit
CACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTG67740.18315260960023577TruSeq Adapter, Index 1 (96% over 25bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA58320.15768320330507454TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT51310.1387298553083569Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC50370.1361883221961009No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC43950.11883019179111842No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT43120.11658607212816896No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT42750.11558568143504692No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGC38620.10441915829290088TruSeq Adapter, Index 1 (97% over 34bp)
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC38340.1036621058764842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)