FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002238570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8518373
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT1901522.232257263212118TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGT635430.745952308028775TruSeq Adapter, Index 1 (96% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT481430.5651666110418033Illumina Single End PCR Primer 1 (96% over 25bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA426470.5006472480132063No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG424830.49872199773360476TruSeq Adapter, Index 1 (97% over 35bp)
AAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTC373130.43802965660226434TruSeq Adapter, Index 1 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTC315500.37037589220382816TruSeq Adapter, Index 1 (96% over 27bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC290570.34110974008769046No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCG249270.29262630316845717TruSeq Adapter, Index 1 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCT230220.2702628776645493TruSeq Adapter, Index 1 (96% over 29bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCC223270.2621040426381892TruSeq Adapter, Index 1 (96% over 33bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC222130.26076575890724674No Hit
GAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTT183600.21553411666758432TruSeq Adapter, Index 1 (96% over 28bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT164610.19324112714951552Illumina Single End PCR Primer 1 (96% over 25bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC148110.17387123104376856No Hit
CACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTG141270.16584152865811347TruSeq Adapter, Index 1 (96% over 25bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT120550.14151763488168456Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA102630.12048075377774606TruSeq Adapter, Index 1 (97% over 36bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC101780.11948291064502575No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT90010.10566571808959292Illumina Single End PCR Primer 1 (96% over 25bp)
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT88860.10431569502767724No Hit
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT86710.10179173886844355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)