FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002238572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002238572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8595014
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTAT1940742.257983523936087TruSeq Adapter, Index 1 (97% over 36bp)
GAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGT650100.7563687505337396TruSeq Adapter, Index 1 (96% over 31bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCATCTCGTAT520260.6053044241696406Illumina Single End PCR Primer 1 (96% over 25bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATG429610.4998363004411628TruSeq Adapter, Index 1 (97% over 35bp)
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA426590.4963226354256084No Hit
AAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTC389580.4532627870065133TruSeq Adapter, Index 1 (96% over 30bp)
AGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTC329600.38347814209494013TruSeq Adapter, Index 1 (96% over 27bp)
GCCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTC297940.34664283269346624No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCG256330.2982310441844539TruSeq Adapter, Index 1 (96% over 32bp)
AGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCT239650.2788244440323192TruSeq Adapter, Index 1 (96% over 29bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCC228190.2654911324170036TruSeq Adapter, Index 1 (96% over 33bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC222240.25856851425721933No Hit
GAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTT189070.21997637234796824TruSeq Adapter, Index 1 (96% over 28bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACATCCTGTAATCTCGTAT175210.2038507441640002Illumina Single End PCR Primer 1 (96% over 25bp)
CACACGTCTGAACTCCAGTCACAACGTGATATCTCGTATGCCGTCTTCTG154720.18001134145912967TruSeq Adapter, Index 1 (96% over 25bp)
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC145440.1692143840603401No Hit
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT127340.14815566327175267Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATATCTCGTA108070.12573568815594716TruSeq Adapter, Index 1 (97% over 36bp)
AATGATACGGCGACCACCGAGAACTCCAGTCACCCTAATCCAGCTCGTAT107320.12486308922824325Illumina Single End PCR Primer 1 (96% over 25bp)
CCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTAC103420.12032557480418297No Hit
GGCGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACT90800.10564264351401872No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGTGATAGCTCGTAT87650.10197772801766233TruSeq Adapter, Index 1 (97% over 36bp)
CTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTT86530.10067464695229118No Hit
AATGATACGGCGACCACCGAGAACTCCAGTCACGCTAACGAATCTCGTAT86350.10046522320964224Illumina Single End PCR Primer 1 (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)