FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002248603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002248603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16585932
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA6748524.068821697809928RNA PCR Primer, Index 1 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4923142.968262501015921RNA PCR Primer, Index 1 (100% over 33bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4130972.4906468928004766RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT3641692.1956499037859314RNA PCR Primer, Index 8 (100% over 36bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT2963221.7865863672900624RNA PCR Primer, Index 8 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGC2394741.4438380671040978RNA PCR Primer, Index 25 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC1668791.0061478607292011RNA PCR Primer, Index 5 (100% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1287860.7764773182477777RNA PCR Primer, Index 1 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC1205250.7266700478453668RNA PCR Primer, Index 5 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1197620.7220697637009486No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC1185980.7150517679681793RNA PCR Primer, Index 25 (100% over 44bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1183860.71377357630551RNA PCR Primer, Index 1 (100% over 34bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTATGCC1148610.6925206253106548RNA PCR Primer, Index 25 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC945400.5700011310790373RNA PCR Primer, Index 5 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC810800.4888480189114486No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC788620.47547524010106873No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC771990.46544867059626194No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA737960.4449312827280372RNA PCR Primer, Index 1 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA648510.391000035451731No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA594730.35857496582043147No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT592960.35750779636622165RNA PCR Primer, Index 25 (100% over 39bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA589870.35564477172582165RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT586790.35378777629137753No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA560580.3379852274807349No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG538060.32440745566785156No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA520480.3138081115972259RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA501260.30221997774981835RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC501100.30212351045452257RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT486730.29345954149576886No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG482200.2907283111977066Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC476170.28709270000624626No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGAT472210.2847051344476753RNA PCR Primer, Index 25 (100% over 39bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG465790.2808343842239315No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA463790.279628543032734RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG460610.27771125553873005No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC454210.2738525637268982RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC452600.2728818615679842RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC446520.269216104346744RNA PCR Primer, Index 1 (100% over 33bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA440860.26580357377565517RNA PCR Primer, Index 25 (100% over 38bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA423790.2555117192087849RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA422150.25452292943200294RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCG410710.24762551781835354RNA PCR Primer, Index 25 (100% over 45bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC397330.23955844024924253No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA393270.23711058263111173RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG390020.23515109069541587Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG381770.23017699578172635No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC379280.2286757234986855RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA378150.22799442322565894RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA365550.220397623721115RNA PCR Primer, Index 25 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG362850.2187697381129984No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC362780.2187275336713065RNA PCR Primer, Index 1 (100% over 27bp)
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA362780.2187275336713065RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC342600.20656059605212418No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC321060.19357368642292758No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT320830.19343501468593985RNA PCR Primer, Index 8 (100% over 36bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC308540.1860251205660315RNA PCR Primer, Index 1 (100% over 26bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT308500.18600100374220754RNA PCR Primer, Index 1 (100% over 25bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT305600.18425253401497124RNA PCR Primer, Index 1 (100% over 30bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGA303360.1829019918808301RNA PCR Primer, Index 25 (100% over 38bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA300350.18108720088807792RNA PCR Primer, Index 1 (100% over 22bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA286950.17300806490705498RNA PCR Primer, Index 1 (100% over 23bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC281770.16988493622185358RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA281760.16987890701589756No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA280140.16890217565102764RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC278900.16815455411248523RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG278520.1679254442861577No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC272200.16411498612197373RNA PCR Primer, Index 1 (100% over 33bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG269430.16244489607216525RNA PCR Primer, Index 5 (97% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT267590.1613355221762636No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA264790.15964734450858714RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA263730.15900824867725252RNA PCR Primer, Index 1 (100% over 32bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTC263670.1589720734415166RNA PCR Primer, Index 25 (100% over 44bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT263120.15864046711393728No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG254760.15360005093473192Illumina Small RNA Adapter 2 (100% over 21bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG245980.14830640810537507RNA PCR Primer, Index 5 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG244410.14735982277028506RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG242280.1460756019016598Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG239320.14429095693868757RNA PCR Primer, Index 1 (100% over 29bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTAT231470.13955803026323754RNA PCR Primer, Index 25 (100% over 48bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT227870.13738751611908212RNA PCR Primer, Index 8 (100% over 36bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG227670.13726693199996237RNA PCR Primer, Index 1 (100% over 29bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG225290.1358319809824374No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC224400.13529538165235455RNA PCR Primer, Index 1 (100% over 24bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA223560.1347889283520516RNA PCR Primer, Index 1 (100% over 32bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT221450.13351676589533829No Hit
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT220250.13279326118061982RNA PCR Primer, Index 1 (100% over 30bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA219950.1326123850019402RNA PCR Primer, Index 1 (100% over 23bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA215520.12994144676343783RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG214750.1294771979048268RNA PCR Primer, Index 5 (97% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT213350.1286331090709886No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTG211780.1276865237358986RNA PCR Primer, Index 5 (97% over 37bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA208320.12560041847512698RNA PCR Primer, Index 25 (100% over 40bp)
TCTCCCAACCCTTGTACCAGTGATGGAATTCTCGGGTGCCAAGGAACTCC207590.1251602864403399RNA PCR Primer, Index 1 (100% over 27bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA201880.12171760983947118RNA PCR Primer, Index 1 (100% over 22bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACT200340.12078911212224915RNA PCR Primer, Index 8 (100% over 36bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC199280.1201500162909145No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA198500.11967973822634749No Hit
TCTGACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATAT195230.11770818787873966RNA PCR Primer, Index 25 (100% over 41bp)
TATTGCACTTGTCCCGGCCTGTATGGAATTCTCGGGTGCCAAGGAACTCC193900.11690630348659334RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCT190520.11486843187346962RNA PCR Primer, Index 25 (100% over 43bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA190350.11476593537221785RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC189720.11438609539699066RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC187310.11293305676159772RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT186580.11249292472681066RNA PCR Primer, Index 1 (100% over 30bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC183730.11077460102935427RNA PCR Primer, Index 5 (100% over 35bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA181880.10965919792749663RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC179510.10823027611592764RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC178130.1073982456940014RNA PCR Primer, Index 5 (100% over 35bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATA176480.10640342671126349RNA PCR Primer, Index 25 (100% over 40bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA175860.10602961594199228RNA PCR Primer, Index 1 (100% over 22bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC175650.10590300261691654RNA PCR Primer, Index 1 (100% over 26bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC175640.10589697341096056No Hit
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACTGATATCTCGTA169210.10202019398126073RNA PCR Primer, Index 25 (100% over 47bp)
TATTGCACTTGTCCCGGCCTGTAATGGAATTCTCGGGTGCCAAGGAACTC167750.1011399299116866RNA PCR Primer, Index 1 (100% over 26bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG167410.10093493690918304No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)