FastQCFastQC Report
Tue 7 May 2024
EGAF00002248676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002248676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459284272
Total Bases68.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG74305171.6178470400571434TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT11777860.2564394367068594TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCCCGTATG9585220.2086990690593472TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)