FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13316817
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA12133349.111291384420166RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT5409704.062307081339332RNA PCR Primer, Index 3 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1562301.1731782452218125RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1442391.083134205418607RNA PCR Primer, Index 3 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1341891.007665720719899No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1292850.970840103907713No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1263160.9485449863882637No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA941540.7070308167484768RNA PCR Primer, Index 3 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC938990.7051159447486588No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC884090.6638898769878718No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT881710.662102663121375RNA PCR Primer, Index 3 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC811120.6090945005852375No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG752520.5650899910992243No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA738890.5548548125276483No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC723690.5434406735483411No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG704740.5292105463340075Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT663180.4980018873879546RNA PCR Primer, Index 1 (97% over 35bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT646680.48561153915383837RNA PCR Primer, Index 1 (100% over 30bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA634070.47614230938218943RNA PCR Primer, Index 1 (100% over 32bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT616470.46292593793246545RNA PCR Primer, Index 1 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC610890.4587357474387461RNA PCR Primer, Index 3 (100% over 50bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA579410.43509646486844417RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT570970.42875861401414467No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA565210.4244332560851441RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG539110.4048339779693601No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT536080.4025586594754587RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG514050.3860156672574235No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT498320.37420353527423256RNA PCR Primer, Index 3 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC490890.36862412391790017No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG465910.34986588762164417No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC448640.33689732313660237RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG447740.336221485960196No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT446010.3349223767211038RNA PCR Primer, Index 3 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA437390.3284493584315231No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC416520.312777445240856RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC412630.3098563267783886RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA407670.30613171300619363No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC406010.3048851688808219RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC392600.29481519495236735No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA377120.2831907955181783RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC362160.2719568797859128No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG361470.271438737950668No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA360870.2709881798330637RNA PCR Primer, Index 1 (100% over 32bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC358850.26947130083712945RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT354840.2664600707511412RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG353060.2651234150022487No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA344040.2583500246342651RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC327430.245877074078588RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC314330.23603988851089566No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT313110.23512375367176708No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA309910.23272077704454447RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA305930.22973207486443648RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA305600.22948426789975415No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC298780.22436292396298607RNA PCR Primer, Index 1 (100% over 24bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA296080.22233541243376703RNA PCR Primer, Index 1 (100% over 32bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC293140.22012767765750627RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT286430.215088936042299No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG285140.2141202360894499RNA PCR Primer, Index 1 (100% over 29bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC281700.21153703621518563RNA PCR Primer, Index 3 (100% over 50bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC279450.2098474432741698RNA PCR Primer, Index 1 (100% over 26bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA277480.2083681107880359RNA PCR Primer, Index 3 (100% over 36bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG277260.20820290614491435RNA PCR Primer, Index 1 (100% over 29bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT272390.20454587609036RNA PCR Primer, Index 1 (100% over 25bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC263300.19771992060865595RNA PCR Primer, Index 1 (100% over 31bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC257130.1930866812992925No Hit
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG256980.1929740417698914RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA253940.19069121397403RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC251940.18918935358201588No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC245730.1845260770648121RNA PCR Primer, Index 1 (100% over 31bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238580.1791569261633617RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC238160.17884153548103876No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC232560.17463632638339927RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA231840.1740956566422742No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA226450.17004814288579623RNA PCR Primer, Index 3 (100% over 36bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA226220.16987542894071458RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG221430.1662784733018408No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT218050.16374032923933698No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC214660.1611946758748731RNA PCR Primer, Index 1 (100% over 24bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA209180.15707957840075448RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA207510.1558255249734227RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA198730.14923235785248082RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC198420.14899956949171864No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC197610.14839131603295294RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGCC197400.14823362069179144No Hit
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA196990.14792573931142855RNA PCR Primer, Index 3 (100% over 36bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA194280.14589071848024945RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC187010.1404314559552782RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC185160.13904223509266517RNA PCR Primer, Index 1 (100% over 26bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA180530.13556542828515253RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC179810.13502475854402746No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG179090.13448408880290239No Hit
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG178060.13371063070101513No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA177220.13307984933636918RNA PCR Primer, Index 1 (100% over 28bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA170430.12798103330548133RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT169140.12701233335263223RNA PCR Primer, Index 1 (100% over 30bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT166950.1253677962233768RNA PCR Primer, Index 1 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG164370.12343039631767862No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC161870.121553070827661RNA PCR Primer, Index 1 (100% over 26bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA159610.11985596858468506RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC157080.11795611518878722RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT152290.11435915954991346RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT151220.11355566424018591No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC151170.11351811773038557RNA PCR Primer, Index 1 (100% over 31bp)
TTTGGCAATGGTAGAACTCACACTGGTGGAATTCTCGGGTGCCAAGGAAC148690.11165581084428809RNA PCR Primer, Index 1 (100% over 24bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC148610.11159573642860754No Hit
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA145200.1090350644602235No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC143140.10748814825644896RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA142910.10731543431136735RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA142640.10711268315844544No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA142530.10703008083688466RNA PCR Primer, Index 1 (100% over 22bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG141190.10602383437423524RNA PCR Primer, Index 3 (100% over 38bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA139560.10479981815474373No Hit
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC138380.10391372052345543RNA PCR Primer, Index 1 (100% over 33bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA138100.10370346006857345RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT137570.1033054670646897No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC137370.1031552810254883No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG136370.10240435082948125No Hit
GTCTACGGCCATACCACCCTGAACGCTGGAATTCTCGGGTGCCAAGGAAC136140.10223163688439962RNA PCR Primer, Index 1 (100% over 24bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC134800.10122539042175017No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT134300.10084992532374665RNA PCR Primer, Index 1 (100% over 25bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT134000.10062464626494454RNA PCR Primer, Index 3 (100% over 34bp)
GTTAAAGACTTTTTCTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCA133310.10010650442969968RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)