FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10332030
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5348565.176678735930887RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG3496153.3837977628791247RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA2728012.640342701289098RNA PCR Primer, Index 3 (97% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2275952.2028100963702197RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA1409461.3641656092752343RNA PCR Primer, Index 3 (97% over 36bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG948020.917554439930972RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC826860.8002880363297435No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT790600.7651932872823637RNA PCR Primer, Index 1 (97% over 35bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG768660.7439583508758685RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA751480.7273304471628518No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT750540.726420654992291RNA PCR Primer, Index 1 (97% over 35bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT746390.7224040193456659RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA665820.6444232159604647No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC515480.4989145405114No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC493280.4774279594619838No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG487300.47164013267479865RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGC468610.45355075430481717RNA PCR Primer, Index 17 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG426380.41267785711036453No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA425190.411526098936995RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG413710.4004150200880176Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC408200.3950820893861129RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA403940.3909589886982519No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC365580.3538317252272786RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA361710.3500860915037993RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC361580.3499602691823388RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA340810.3298577336689886RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC338770.3278832910860693RNA PCR Primer, Index 1 (100% over 24bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA333600.3228794341479845RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG332690.32199867789776065No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA321750.31141024561485015RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG299440.28981719952419804No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA293440.2840100154567883RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT291770.2823936825580259No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAGATCTCGTATGCC290320.2809902797417352RNA PCR Primer, Index 17 (100% over 50bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA289970.2806515273378029RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC284570.2754250616771341RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC265490.25695821634277094No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA265060.25654203481793997RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT260950.25256411373176424RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT257240.2489733382500825RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC254980.24678596558469149No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA248190.24021416894840605RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT245870.23796872444234096RNA PCR Primer, Index 1 (100% over 30bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC245680.2377848302802063RNA PCR Primer, Index 1 (100% over 33bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC244120.23627496242267979RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG238000.23035163467392178No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC235730.2281545833684184No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC235170.22761257952212682RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA231270.22383790987831045RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG222430.21528199201899337No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA216400.2094457720312465No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA216010.20906830506686488RNA PCR Primer, Index 3 (97% over 36bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGA212620.20578724606877835RNA PCR Primer, Index 17 (100% over 38bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA210960.20418059181012832RNA PCR Primer, Index 1 (100% over 23bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG208510.20180932498260265RNA PCR Primer, Index 1 (100% over 29bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA208070.20138346481765926No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC207230.20057045904822188RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA206720.20007684840249204RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA202200.19570210307171002RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC199340.19293401199957802RNA PCR Primer, Index 1 (100% over 31bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC197710.191356393661265RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG196390.19007881316643488RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA194210.18796886962194262RNA PCR Primer, Index 1 (100% over 28bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG191360.185210457189923RNA PCR Primer, Index 3 (97% over 37bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG186440.18044856625464695RNA PCR Primer, Index 17 (100% over 39bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA185770.17980009736711952No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT181980.17613189276453903No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA181820.17597703452274144RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGAG172750.1671985079408403RNA PCR Primer, Index 17 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC172140.16660811089398694No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA171860.16633710897084117No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC169820.16436266638792182No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT169200.16376259070095617No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA167940.1625430820468001RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG160420.15526474468231316No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAGA159170.15405491466826945RNA PCR Primer, Index 17 (100% over 38bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA156030.15101582167299166RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC153140.14821869468052262RNA PCR Primer, Index 1 (100% over 24bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA151410.14654428994108612RNA PCR Primer, Index 3 (97% over 36bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT150700.1458571064931093RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC150130.14530542400670535RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA149690.14487956384176195RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA146340.14163721940412485RNA PCR Primer, Index 3 (97% over 36bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG143370.138762663290757RNA PCR Primer, Index 3 (97% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC141810.13725279543323046No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC140350.1358397139768274RNA PCR Primer, Index 1 (100% over 33bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA140210.1357042130152545No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA140080.13557839069379396RNA PCR Primer, Index 1 (100% over 28bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT139990.1354912829327828RNA PCR Primer, Index 1 (97% over 35bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA139760.13526867421019878RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA137870.1334394112289647No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG137410.1329941937837966No Hit
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTAG136990.1325876908990779RNA PCR Primer, Index 3 (97% over 37bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA132690.12842587565076757No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG129330.12517385257301808No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG129320.12516417393290574No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT129130.12498027977077109No Hit
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT128970.1248254215289735RNA PCR Primer, Index 1 (97% over 35bp)
GTTAAAGACTTTTTCTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCA126740.12266708478391951RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC126730.12265740614380717RNA PCR Primer, Index 1 (100% over 31bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTA124970.12095396548403364RNA PCR Primer, Index 3 (97% over 36bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA123440.11947313354684413RNA PCR Primer, Index 1 (100% over 28bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA122950.11899888018133901RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG118810.11499192317482625RNA PCR Primer, Index 1 (100% over 29bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA117800.11401438052347894RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT116620.11287230099022168No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA116390.11264969226763763RNA PCR Primer, Index 1 (100% over 22bp)
CTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115270.11156568457505447RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC115180.11147857681404333RNA PCR Primer, Index 1 (100% over 26bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG113910.11024938951977492No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC113850.11019131767910081RNA PCR Primer, Index 1 (100% over 27bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA113450.10980417207460683RNA PCR Primer, Index 1 (100% over 32bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG113210.10957188471191043RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG113070.10943638375033754No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT111260.10768454989000226No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC110200.1066586140380932RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC108840.10534231898281364RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC107380.10392923752641059RNA PCR Primer, Index 1 (100% over 26bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT105500.10210965318528885RNA PCR Primer, Index 1 (100% over 30bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC105290.1019064017429295No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG105010.10163539981978371No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA104560.10119986101472797RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)