FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9741719
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7465697.663626922517473RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2988423.067651612615802RNA PCR Primer, Index 1 (100% over 33bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2966153.044791170839561RNA PCR Primer, Index 1 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG2696512.7680022386192826RNA PCR Primer, Index 15 (100% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG2127482.1838856160806936RNA PCR Primer, Index 15 (100% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGC1961462.013463948200518RNA PCR Primer, Index 26 (100% over 50bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTATGCC1604381.6469167299939569RNA PCR Primer, Index 26 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1037251.064750481922133RNA PCR Primer, Index 1 (100% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT864120.8870303074847468RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA779970.8006492488645998RNA PCR Primer, Index 1 (100% over 34bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC753610.7735903694204277RNA PCR Primer, Index 26 (100% over 44bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA666490.6841605675548638RNA PCR Primer, Index 1 (100% over 34bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA607120.623216498032842RNA PCR Primer, Index 1 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT585470.600992494240493RNA PCR Primer, Index 1 (100% over 35bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA515060.5287157225536889RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA479410.49212053847991305No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC473330.48587934018626483No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA451910.46389143435568203RNA PCR Primer, Index 1 (100% over 32bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC351200.36051132248836165RNA PCR Primer, Index 26 (100% over 39bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC324910.33352429894559676RNA PCR Primer, Index 1 (100% over 27bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCG319470.32794006889338523RNA PCR Primer, Index 26 (100% over 45bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG314670.3230128070826104RNA PCR Primer, Index 26 (100% over 38bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA311500.31975876126174446No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGC278480.28586330605512233RNA PCR Primer, Index 26 (100% over 39bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC274060.28132611913770045RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC257270.2640909679287608No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC256050.2628386222185222RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC250560.25720306652244845No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA248730.25532454795709053RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG243330.2497813784199688RNA PCR Primer, Index 26 (100% over 38bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC241710.24811842755883226RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA240560.2469379377500008No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT236180.2424418113476687No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA233820.24001924095737107RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC233550.23974208248051498RNA PCR Primer, Index 1 (100% over 24bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA230020.23611849202384094RNA PCR Primer, Index 1 (100% over 32bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA224500.23045214094144986RNA PCR Primer, Index 1 (97% over 37bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC221780.2276600259153441No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG221450.22732127666585333Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC220500.2263460894324708RNA PCR Primer, Index 1 (100% over 31bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC218640.22443677548079555RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC211500.21710747353726792RNA PCR Primer, Index 1 (100% over 27bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTC206230.211697750674188RNA PCR Primer, Index 26 (100% over 44bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA202330.2076943504529334RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG199590.20488170516928275Illumina Small RNA Adapter 2 (100% over 21bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT193150.19827096223982646RNA PCR Primer, Index 1 (100% over 25bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA189890.19492453026000853RNA PCR Primer, Index 1 (97% over 37bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT189620.19464737178315245RNA PCR Primer, Index 1 (100% over 30bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG186570.1915165075075559RNA PCR Primer, Index 15 (100% over 36bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTA186560.19150624237878347RNA PCR Primer, Index 26 (100% over 47bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG186470.19141385621983142No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC183030.18788265192210943RNA PCR Primer, Index 1 (100% over 26bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAG182090.18691772981749935RNA PCR Primer, Index 26 (100% over 38bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG180130.1849057645780996RNA PCR Primer, Index 15 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC178110.1828322085660652No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC174150.17876721757217592No Hit
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCTCGTAT173470.17806918881564948RNA PCR Primer, Index 26 (100% over 48bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA173430.1780281283005597RNA PCR Primer, Index 1 (100% over 23bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC171080.1756158230390345RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG169930.174435333230203Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA169030.1735114716406827No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC167550.17199223258236046RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG167080.17150977153005542RNA PCR Primer, Index 1 (100% over 29bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG161560.1658434204476643RNA PCR Primer, Index 15 (100% over 36bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT161510.16579209480380208RNA PCR Primer, Index 1 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT159000.16321554748191772No Hit
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA157540.16171683868114037RNA PCR Primer, Index 1 (100% over 32bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA155370.15948930573751924RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA154650.15875021646590298RNA PCR Primer, Index 1 (100% over 28bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA154620.15871942107958564RNA PCR Primer, Index 26 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT151450.15546537525871976No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG149980.15395640132916993No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT147920.15184178480204572RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA145550.14940894928297563RNA PCR Primer, Index 26 (100% over 40bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT145350.14920364670752667RNA PCR Primer, Index 1 (100% over 35bp)
TCTGACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCAT143750.14756122610393504RNA PCR Primer, Index 26 (100% over 41bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA141380.14512839058486496RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA138750.14242866171771124RNA PCR Primer, Index 1 (97% over 37bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC137350.14099154368956854RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC136640.14026271954672476RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC134670.13824048917855256No Hit
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA134360.13792227018660672RNA PCR Primer, Index 1 (100% over 32bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT134070.1376245814522057No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC131170.1346476941081959No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG129160.13258440322493392Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG128750.13216353294526356RNA PCR Primer, Index 1 (100% over 29bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG126680.1300386512893669RNA PCR Primer, Index 15 (100% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGA126670.13002838616059445RNA PCR Primer, Index 1 (97% over 37bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG126530.12988467435778017No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC126130.1294740692068823RNA PCR Primer, Index 1 (100% over 31bp)
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA126090.1294330086917925RNA PCR Primer, Index 26 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG124710.12801642092119472No Hit
GTAGAATTCTCGCCTCCCACGCGGGAGACCCGGGTTTGGAATTCTCGGGT122900.1261584326133817No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC122580.12582994849266335RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA120930.12413620224520948RNA PCR Primer, Index 1 (100% over 28bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC118430.12156992005209759No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG116140.11921920556320707No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG114440.11747413367189097No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCATCT114300.11733042186907669RNA PCR Primer, Index 26 (100% over 43bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA114280.1173098916115318RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC113690.1167042490139574No Hit
GTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATG113440.1164476207946462RNA PCR Primer, Index 15 (100% over 36bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT112770.11575985716689222RNA PCR Primer, Index 1 (100% over 25bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA112680.11566747100794018RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG110300.11322437036009764RNA PCR Primer, Index 1 (100% over 29bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT107380.11022695275854293No Hit
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA107170.11001138505432152RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA107050.10988820350905215RNA PCR Primer, Index 1 (100% over 23bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATGAGCA105880.10868718344267578RNA PCR Primer, Index 26 (100% over 40bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC105660.10846135060968193RNA PCR Primer, Index 1 (100% over 27bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA105210.10799941981492178RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA104120.10688052077872498No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC101480.10417052678279881RNA PCR Primer, Index 1 (100% over 31bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC100520.10318507442064384No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA99110.10173769126372871RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)