FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9522444
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7469107.843679626784889RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1765531.8540723368916636No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1753491.8414285240217745RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGC1751241.8390656852379494RNA PCR Primer, Index 32 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC1692111.7769702819990332RNA PCR Primer, Index 30 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT1326831.393371281574352No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1091711.146459879417511RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC934310.981166179606832No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT888790.9333633256336293RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC837210.8791965592026585RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC834440.8762876421221274No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT808410.8489522227697006No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC805240.8456232454609343No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG714640.750479603765588No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC645350.6777146707294892RNA PCR Primer, Index 2 (100% over 34bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA629730.661311318816892RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA583300.6125528278244535No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAA529170.5557081774384811RNA PCR Primer, Index 32 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG471830.4954925437209187No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC467890.4913549504727988No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA454990.47780800811220314RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG452320.475004106088731No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA430130.4517012649273653RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG422650.443846138659361No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC405950.4263086241305278RNA PCR Primer, Index 30 (100% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA399710.41975568457005363RNA PCR Primer, Index 7 (100% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC396720.4166157343639931RNA PCR Primer, Index 1 (100% over 27bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC356390.37426316185214636RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC352630.3703145957067324No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC351620.36925394363043773RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG338060.3550139018932535Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA332240.34890202557242656RNA PCR Primer, Index 7 (100% over 35bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC330700.34728479369371984RNA PCR Primer, Index 1 (100% over 24bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA330660.3472427876708962RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG325870.3422125664377758No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC324670.3409523857530693RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC317500.3334228061619475No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTTGGAATTCT316680.33256168269406466No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA316420.3322886435457116RNA PCR Primer, Index 1 (100% over 32bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG312170.32782550362070917No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC288760.3032414787632251RNA PCR Primer, Index 1 (100% over 27bp)
TCGGATCCGTCTGAGCTTGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA286190.3005425917968118RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC267350.28075775504691863RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA263500.2767146753501517RNA PCR Primer, Index 1 (100% over 23bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG262920.2761055880192102Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG262250.27540198713691566No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC259050.2720415053110315RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC258400.2713589074401488RNA PCR Primer, Index 1 (100% over 27bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA258330.27128539690020753RNA PCR Primer, Index 7 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT253660.2663811937355578No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT248980.2614664890652022RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCAATCTCGTATGCC241650.2537688853827862RNA PCR Primer, Index 32 (100% over 50bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG232940.24462207391295765No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA229880.24140861316695586RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG221970.23310192215359837Illumina Small RNA Adapter 2 (100% over 21bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG219040.23002498098177318No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA212210.2228524525846516No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG207150.2175386906974722No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC203010.21319106733523452RNA PCR Primer, Index 2 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC193990.20371870918852344No Hit
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC191070.20065226952240411RNA PCR Primer, Index 1 (100% over 26bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC189470.198972028609462No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA188930.1984049473013441RNA PCR Primer, Index 1 (100% over 22bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA185580.19488694288987155RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC178100.18703181662186724RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA175370.1841649055641598No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA174550.18330378209627696RNA PCR Primer, Index 7 (100% over 35bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA170250.17878813464274507RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA166540.17489207602586057RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA165300.17358988931833047RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA159740.16775105214585667RNA PCR Primer, Index 1 (100% over 32bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA158060.16598679918726747RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT153630.16133463215955904No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA150450.15799515334508663RNA PCR Primer, Index 1 (100% over 23bp)
TCGTGGGTTCGTGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG149120.1565984530862035No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG146900.15426711881949634RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG146670.1540255841882609RNA PCR Primer, Index 1 (100% over 29bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA137550.14444821098449095RNA PCR Primer, Index 1 (100% over 32bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA137540.14443770947878506RNA PCR Primer, Index 1 (100% over 28bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA137300.14418567334184376RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT136950.14381812064213767RNA PCR Primer, Index 1 (100% over 30bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA134780.14153929390395995RNA PCR Primer, Index 1 (100% over 28bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC133450.14014259364507684RNA PCR Primer, Index 1 (100% over 33bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACTCA132540.13918695662584102RNA PCR Primer, Index 32 (100% over 39bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG131920.13853586327207595No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA129230.13571095823719204RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT127920.1343352609897207RNA PCR Primer, Index 1 (100% over 30bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC127870.13428275346119126RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG126360.13269702609960216No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC126190.13251850050260205RNA PCR Primer, Index 2 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA125110.13138433788636614No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA124210.1304392023728362RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC124070.13029218129295378RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA119780.12578703534512778RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC116210.12203799780812571RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA114620.12036825840088952RNA PCR Primer, Index 1 (100% over 32bp)
ACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT110350.1158841154644753RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC109660.11515951157076902RNA PCR Primer, Index 1 (100% over 26bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG109110.11458192875694517RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA106640.1119880568475908No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC106610.11195655233047315RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG104010.10922616084694224Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA103990.10920515783553045RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC103970.1091841548241187No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATTGGAATTCTCGGGTGCCAAGGA102370.10750391391117659RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT101680.1067793100174703No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC100570.10561364288411673RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC100510.10555063384988139RNA PCR Primer, Index 1 (100% over 27bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG100400.10543511728711662RNA PCR Primer, Index 1 (100% over 29bp)
CGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG100070.10508856759882232RNA PCR Primer, Index 1 (100% over 29bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG98930.10389139594835106No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG98690.10363935981140976No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC97630.10252620020658562RNA PCR Primer, Index 30 (100% over 36bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96480.10131852705040849RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)