FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002258436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20162659
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9325004.6248860331368RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC5222132.59000065417959RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG4904282.4323577559884337RNA PCR Primer, Index 15 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA3056781.5160599601471214No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2960411.468263684864184RNA PCR Primer, Index 6 (100% over 34bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG2150251.0664516024399362RNA PCR Primer, Index 15 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2009640.9967137766898702No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT1982310.983159016873717No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1919190.9518536220842697No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1882650.9337310123630023No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1721450.853781239865238RNA PCR Primer, Index 6 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC1554410.770935023996587No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1543860.7657025792084269No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1392700.6907323086702006RNA PCR Primer, Index 1 (97% over 35bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1389430.6891104987690364RNA PCR Primer, Index 1 (97% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT973260.4827041909502115RNA PCR Primer, Index 1 (97% over 35bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC937850.46514202318255743No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG932490.4624836436503737RNA PCR Primer, Index 6 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC906930.44980674423943784RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC866430.42972010784887055No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC843500.418347599887495No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC808900.40118716484765227No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA794600.3940948463196248No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG783550.388614418366149RNA PCR Primer, Index 6 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC718450.3563270102420519No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG686020.3402428221396791Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC684250.33936496173446173RNA PCR Primer, Index 1 (100% over 27bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC664220.3294307561319169RNA PCR Primer, Index 20 (100% over 50bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC653420.32407431976109896RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG633760.3143236217008878Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG631060.3129845126081833No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG597720.296448995144936No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC567960.2816890371453487No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA563220.27933815673815643RNA PCR Primer, Index 1 (100% over 32bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA559620.2775526779478838RNA PCR Primer, Index 1 (100% over 28bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTCTGGAATTCTCGGGTGC551790.27366926157904076No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC547050.2713183811718484RNA PCR Primer, Index 1 (100% over 31bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC540110.2678763748372673RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA539030.2673407312001855RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA537000.26633391954900393RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT525870.26081381428907763RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA521890.2588398682931651RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC517160.2564939475492791No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA514050.25495149226101577No Hit
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT495890.2459447436967515No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT493630.24482385978952478No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC492170.2440997489468031No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA477150.2366503346607211No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT470460.23333231990879774No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA470340.23327280394912198No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC468230.23222631499148996No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA462100.22918604138472015RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC453890.22511415781023722RNA PCR Primer, Index 1 (100% over 24bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG450020.22319476811069414No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT446290.22134481369743944No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT443590.22000570460473493RNA PCR Primer, Index 1 (100% over 30bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC440070.21825990312091276RNA PCR Primer, Index 20 (100% over 39bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT434290.21539321772986392No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGGAATTCTCGGGTGC430950.21373669018555536No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGCC413580.20512175502248983RNA PCR Primer, Index 20 (100% over 50bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC411250.2039661534721189RNA PCR Primer, Index 1 (100% over 26bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA407250.20198228814959376RNA PCR Primer, Index 1 (100% over 28bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC402660.19970580269199614RNA PCR Primer, Index 1 (100% over 27bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC398500.19764258275656996No Hit
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA394300.19555952416791855No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA390270.19356077985547443No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG390050.19345166726273552No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT386000.1914430036236788No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG380310.18862095520238675No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA377000.18697930664799717No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC371040.18402334731743467No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG364060.18056150232962825No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC358220.1776650589587415RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA357740.17742699512003848RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC354610.17587462050516256No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG349940.1735584577411144No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT345830.17152003612221978No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC341610.16942705820695575No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA340960.1691046800920454RNA PCR Primer, Index 1 (100% over 28bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC338650.1679589978682871No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG319690.15855547623951782Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC314740.1561004429028929RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC311460.15447367333842227No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT303550.15055057966312874No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG302390.14997525871959647No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG298380.14798643373376497No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC296960.14728216154426854No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG279970.1388556935868429Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC273010.1354037679256491RNA PCR Primer, Index 1 (100% over 33bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC271370.1345903831434138No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC267140.1324924455648434No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATTGGAATT266380.13211551115356363No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA261530.12971007445000185RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC258000.12795931330287338RNA PCR Primer, Index 1 (100% over 24bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTTGGAAT253690.12582169841785254No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA252330.12514718420819396No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC250140.12406101794411144RNA PCR Primer, Index 20 (100% over 38bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA249990.12398662299451674RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC248510.12325259282518243RNA PCR Primer, Index 20 (100% over 38bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA247690.12284590043406478RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC246820.12241440972641554No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTTGGAATTCTCGGGTGC245610.12181429046635169No Hit
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA243130.12058429396638608RNA PCR Primer, Index 1 (100% over 28bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC243040.12053965699662926RNA PCR Primer, Index 20 (100% over 38bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTGTGTGGAATTCTCGGGT241610.11983042514382652No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG239720.11889304877893338RNA PCR Primer, Index 15 (97% over 36bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238110.118094542986617RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT232210.11516834163589236No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC232090.11510882567621662No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG228690.11342254015207023RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTGC227880.11302080742425888No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC227850.11300592843433994RNA PCR Primer, Index 20 (100% over 39bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT221620.10991605819450699No Hit
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG218870.10855215078527092RNA PCR Primer, Index 17 (97% over 37bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTCTGGAATTCTCGGGTGC211890.10509030579746452No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG210880.10458937980352692RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC210750.10452490418054484No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT208090.1032056337410656RNA PCR Primer, Index 1 (100% over 30bp)
AGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGG207110.10271958673704694No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC206500.10241704727536184RNA PCR Primer, Index 1 (100% over 26bp)
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA206170.10225337838625352No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG205400.10187148431166744No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG205350.10184668599513585RNA PCR Primer, Index 17 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)