FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12595161
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2879912.286520990085002RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC1688591.3406656731104905RNA PCR Primer, Index 9 (100% over 50bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1409891.119390216607791RNA PCR Primer, Index 6 (100% over 34bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1191060.9456488884897939RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA950520.754670781897905No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT853380.6775459241846928RNA PCR Primer, Index 9 (100% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC723950.5747842365810171RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG722180.5733789349735188Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC680000.5398898831066947RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG671170.5328792541834122No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC639410.5076632208194878No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA595210.4725703784175526RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC568880.4516655245613772No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC565190.44873582798981293RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA558110.4431146215598197No Hit
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT546860.43418261981724565RNA PCR Primer, Index 9 (100% over 36bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC526260.41782713218195466RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT501770.3983831568330092RNA PCR Primer, Index 9 (100% over 36bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT487100.3867358265606926RNA PCR Primer, Index 9 (100% over 36bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA480930.38183711982720986RNA PCR Primer, Index 7 (97% over 35bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA445160.3534373240643768RNA PCR Primer, Index 1 (100% over 32bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA427890.3397257089448877RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA424500.33703419908645865RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG406120.3224412931283689No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC396350.3146843458372624No Hit
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA377830.2999802860797095RNA PCR Primer, Index 7 (97% over 35bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC372250.2955500132153928RNA PCR Primer, Index 9 (100% over 37bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA326990.2596155777603796RNA PCR Primer, Index 1 (100% over 23bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA323170.25658266694645665RNA PCR Primer, Index 7 (97% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA294460.23378819849940785RNA PCR Primer, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC293280.23285133076107561No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT286530.2274921297155312RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA283180.22483237808552028No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA277320.22017979762227732RNA PCR Primer, Index 1 (100% over 28bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG276380.21943347925445336No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT276230.2193143858978857RNA PCR Primer, Index 1 (100% over 30bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC266200.21135101012206195RNA PCR Primer, Index 1 (100% over 27bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG261320.20747650625506098No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA258550.20527724893711166RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC257260.2042530460706298RNA PCR Primer, Index 1 (100% over 24bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA253770.20148214064115577RNA PCR Primer, Index 1 (100% over 23bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA252150.20019593239022512RNA PCR Primer, Index 9 (100% over 40bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT251040.19931464155162446RNA PCR Primer, Index 1 (100% over 30bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG247940.1968533788492263RNA PCR Primer, Index 6 (100% over 34bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG241580.19180382053075778RNA PCR Primer, Index 1 (100% over 29bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA238790.18958868409859944No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG238340.1892314040288965RNA PCR Primer, Index 1 (100% over 29bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA234870.18647637771363146RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC229630.1823160497908681RNA PCR Primer, Index 1 (100% over 26bp)
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA228620.18151415452331257RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCA228550.18145857762358097RNA PCR Primer, Index 9 (100% over 38bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC227790.1808551712836382RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG226930.18017236937265035RNA PCR Primer, Index 6 (100% over 34bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG226390.17974363328900678RNA PCR Primer, Index 6 (100% over 34bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA225260.17884646333619714RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC224170.17798105161180552RNA PCR Primer, Index 1 (100% over 27bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG211840.16819157770194443RNA PCR Primer, Index 6 (100% over 34bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA211580.16798514921722715RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC210290.16696094635074535RNA PCR Primer, Index 1 (100% over 33bp)
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC209550.16637341912501158RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG207100.1644282276344066No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG205470.1631340798263714No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA203320.16142707504890172RNA PCR Primer, Index 1 (100% over 23bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA202580.16083954782316795RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA199860.15867998829074118RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC199450.15835446644945625No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA199320.15825125220709765RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG196720.156186967359925No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC193060.15328108945967422RNA PCR Primer, Index 1 (100% over 31bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG191330.15190754608059398RNA PCR Primer, Index 1 (100% over 29bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA190260.1510580134704114RNA PCR Primer, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT189590.1505260631444092No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA189120.1501529039604972RNA PCR Primer, Index 1 (100% over 22bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA187770.14908106375138833RNA PCR Primer, Index 1 (100% over 32bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCG183640.1458020266672256No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC182900.14521449944149184RNA PCR Primer, Index 1 (100% over 31bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG182210.14466667000128064RNA PCR Primer, Index 6 (100% over 34bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG176830.14039518827905414RNA PCR Primer, Index 6 (100% over 34bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA176120.13983147972463394RNA PCR Primer, Index 1 (100% over 22bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA174850.13882315597236114RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA173100.13743373347907184RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC172240.13675093156808396RNA PCR Primer, Index 9 (100% over 50bp)
TCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCGGGTGCCAAG172110.1366477173257253No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC168330.13364656474022046RNA PCR Primer, Index 9 (100% over 37bp)
TTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG166360.13208247199063197RNA PCR Primer, Index 9 (100% over 39bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG164300.13044692322710286RNA PCR Primer, Index 9 (100% over 39bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA163340.12968472574506987RNA PCR Primer, Index 7 (97% over 35bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA161740.12841439660834825RNA PCR Primer, Index 9 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC158030.12546882092257494No Hit
CTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA155700.12361890411722407RNA PCR Primer, Index 7 (97% over 35bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA155440.12341247563250679RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA154110.12235651453760694RNA PCR Primer, Index 1 (100% over 28bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC149270.118513768899024RNA PCR Primer, Index 1 (100% over 31bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT148160.11763247806042337RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG146650.11643360493764232No Hit
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC146030.11594135239716269RNA PCR Primer, Index 1 (100% over 33bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG144110.11441695743309674No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA141460.1123129748004015No Hit
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT140900.11186835960254894RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA140320.11140786529048735RNA PCR Primer, Index 1 (100% over 22bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC138060.10961352538486804RNA PCR Primer, Index 1 (100% over 33bp)
CCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA137580.10923242664385155RNA PCR Primer, Index 7 (97% over 35bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG136940.1087242949891629RNA PCR Primer, Index 9 (100% over 39bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT136910.10870047631784936RNA PCR Primer, Index 1 (100% over 25bp)
ATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCCAG136690.10852580606155016RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCT136430.10831937757683288RNA PCR Primer, Index 9 (100% over 43bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT134340.10666001014199024No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG132160.10492918669320701No Hit
ATCCCGGACGAGCCCCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCA131670.10454014839508602RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC131550.1044448737098319RNA PCR Primer, Index 1 (100% over 33bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC128810.10226943506319609RNA PCR Primer, Index 1 (100% over 24bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAG128290.10185657809376157RNA PCR Primer, Index 9 (100% over 39bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG126610.10052273250020385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)