FastQCFastQC Report
Sat 11 May 2024
EGAF00002258442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56363266
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA943510.167398035450962No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG876380.15548779589883951TruSeq Adapter, Index 18 (97% over 39bp)
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG855680.1518151911211107No Hit
CTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGG810400.14378158994547974No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG717760.12734535291123833No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC675470.11984223909239042No Hit
CTTCATTGCGCCACGGCGGCTTTCGTGCGAGCCCCCGACTCGCGCACGTG656940.11655463684450082No Hit
CGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAG580480.10298906383459042No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)