FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15527373
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA8258585.3187232637484785RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5219943.361766346438641RNA PCR Primer, Index 2 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA3000661.9324968879152964RNA PCR Primer, Index 2 (100% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2430891.5655513653211008RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA2021101.3016367932940105RNA PCR Primer, Index 2 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1635321.0531852361632583No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1600931.0310372527278118RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1217560.7841377933021896No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1136860.7321650610183705No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1074180.6917976402061057RNA PCR Primer, Index 2 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1030900.6639242839081665No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1013140.6524864186620621No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG1012120.651829514239144RNA PCR Primer, Index 2 (100% over 35bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG986400.6352652183985018RNA PCR Primer, Index 2 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG951920.6130592728080919Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC950820.6123508464696508RNA PCR Primer, Index 2 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC914580.589011418737735No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC912370.5875881258214123No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC768120.49468767189401586RNA PCR Primer, Index 2 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG675860.43527002281712435No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT669710.4313092755612942No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC655360.42206753196435737RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC637630.4106489874365741RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC597170.38459177866082045RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG587440.37832542568533645No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA580870.37409418837301067RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG558770.3598612592097839No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA537930.3464398002160443RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG510610.3288450660649422No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA494720.31861152559418776RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC485280.3125319395624746RNA PCR Primer, Index 1 (100% over 26bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT450470.29011346607053234RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC439950.2833383341792588No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA432180.2783342681340881RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC416730.2683840981987101RNA PCR Primer, Index 1 (100% over 24bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC416570.26828105436766414RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA406270.2616476077440788RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG395810.2549111172894475No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGCC390840.25171031828758156RNA PCR Primer, Index 2 (100% over 50bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA388080.24993281220203828RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG383400.2469187801439432RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC360200.2319774246422753No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA358810.2310822313600633RNA PCR Primer, Index 2 (100% over 36bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG357110.2299873906551997No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT344240.22169880249543825No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC340690.21941251749410542RNA PCR Primer, Index 1 (100% over 26bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG339060.21836275846532444RNA PCR Primer, Index 2 (100% over 38bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA328680.2116777899262161No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG327420.21086631975672895RNA PCR Primer, Index 1 (100% over 29bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA323400.2082773435016986RNA PCR Primer, Index 2 (100% over 36bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC322740.20785228769863387RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT317470.20445828151355672RNA PCR Primer, Index 2 (100% over 37bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC314140.20231368177991213RNA PCR Primer, Index 1 (100% over 33bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA305710.1968845599316768RNA PCR Primer, Index 1 (100% over 28bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT296750.19111410539310159RNA PCR Primer, Index 2 (100% over 39bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC294800.18985825870222864No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA292450.18834480243374074RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT291400.18766857729250144No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC290080.18681846568637206RNA PCR Primer, Index 1 (100% over 31bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA281410.1812347780915677No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC279690.18012705690782338RNA PCR Primer, Index 1 (100% over 24bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT276610.17814346316018814RNA PCR Primer, Index 1 (100% over 25bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA272570.175541606426277RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC271520.17486538128503773RNA PCR Primer, Index 1 (100% over 26bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC270730.17435660236924816RNA PCR Primer, Index 1 (100% over 27bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA270200.17401526967890835RNA PCR Primer, Index 1 (100% over 32bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA270120.17396374776338536RNA PCR Primer, Index 2 (100% over 36bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA257920.16610665564612892RNA PCR Primer, Index 1 (100% over 32bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC257850.16606157397004634RNA PCR Primer, Index 1 (100% over 27bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC246350.1586552986136161RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA246340.15864885837417572RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC232890.14998673632687254RNA PCR Primer, Index 1 (100% over 31bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC230850.14867292748103625RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT230100.14818990952300817No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGT227590.14657340942347427RNA PCR Primer, Index 2 (100% over 39bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA219990.14167882744878996RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT219790.14155002265998248RNA PCR Primer, Index 2 (100% over 37bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG214430.13809805431994193No Hit
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC213690.13762147660135426RNA PCR Primer, Index 1 (100% over 31bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT211100.13595345458629737RNA PCR Primer, Index 1 (100% over 30bp)
GTTAAAGACTTTTTCTCTGACCTGGAATTCTCGGGTGCCAAGGAACTCCA207400.13357056599335895RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA203550.1310910738088149RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA203520.1310717530904938RNA PCR Primer, Index 2 (100% over 36bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA200790.12931356772327166No Hit
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT200780.1293071274838313RNA PCR Primer, Index 2 (100% over 37bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC200300.12899799599069334RNA PCR Primer, Index 1 (100% over 33bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTC196690.1266730695527183RNA PCR Primer, Index 2 (100% over 44bp)
GTCTACGGCCATACCACCCTGAACGCTGGAATTCTCGGGTGCCAAGGAAC195800.126099888242525RNA PCR Primer, Index 1 (100% over 24bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA192290.12383936419895368RNA PCR Primer, Index 1 (100% over 28bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG191020.12302145379002617No Hit
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA190980.12299569283226468RNA PCR Primer, Index 2 (100% over 40bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA190680.12280248564905344RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA182580.11758589170235044RNA PCR Primer, Index 1 (100% over 23bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT182050.1172445590120106RNA PCR Primer, Index 1 (100% over 30bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC180740.11640088764532158RNA PCR Primer, Index 1 (100% over 26bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC180320.11613039758882587RNA PCR Primer, Index 1 (100% over 33bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG179250.11544129196870585RNA PCR Primer, Index 2 (100% over 38bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG178390.11488743137683366RNA PCR Primer, Index 2 (100% over 35bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTA178010.11464270227809946RNA PCR Primer, Index 2 (100% over 40bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG177490.1143078098272RNA PCR Primer, Index 2 (100% over 35bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG176480.11365734564372221RNA PCR Primer, Index 2 (100% over 35bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC175290.1128909571503177No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT174460.11235641727676665RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA172280.11095244507876509No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC171630.11053382951514079RNA PCR Primer, Index 1 (100% over 27bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC171380.11037282352913141RNA PCR Primer, Index 1 (100% over 26bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC169830.10937458641587343No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATG168500.10851803457030368RNA PCR Primer, Index 2 (100% over 38bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC168420.10846651265478068RNA PCR Primer, Index 2 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA168040.10822178355604647RNA PCR Primer, Index 1 (100% over 28bp)
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA165520.1065988432170722RNA PCR Primer, Index 1 (100% over 23bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG164910.10620598861120938RNA PCR Primer, Index 1 (100% over 29bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGAT160830.10357837091953673RNA PCR Primer, Index 2 (100% over 37bp)
CCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC159150.10249641069355389RNA PCR Primer, Index 2 (100% over 34bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA158970.10238048638362715RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCG157460.10140801022813067RNA PCR Primer, Index 2 (100% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA156030.10048705598815719RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)