FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11693933
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGC8434197.212449395767874RNA PCR Primer, Index 7 (100% over 50bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7306916.248462343678556RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG4615313.9467559802164076RNA PCR Primer, Index 7 (100% over 36bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATGCC3964223.3899800862549836RNA PCR Primer, Index 7 (100% over 50bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3253832.7824941360618363RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG2603842.2266589008163464RNA PCR Primer, Index 7 (100% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC2037711.742536065496527RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1311441.1214704240224396RNA PCR Primer, Index 7 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1251651.0703413470899825RNA PCR Primer, Index 2 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1099960.9406245101626629RNA PCR Primer, Index 7 (100% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC1094360.9358357021542709RNA PCR Primer, Index 7 (100% over 44bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA1034970.8850486829366989RNA PCR Primer, Index 7 (100% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA908110.7765650786608749No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA696790.5958559878870522RNA PCR Primer, Index 1 (100% over 28bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA682730.5838326592088393No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC599280.5124708684409257RNA PCR Primer, Index 2 (100% over 34bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC581210.49701841117098927RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC539030.4609484251363506RNA PCR Primer, Index 2 (100% over 34bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG491080.41994425656449375RNA PCR Primer, Index 7 (100% over 45bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC485380.4150699341273804No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA455770.3897491117830075No Hit
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTA385190.3293930279915235RNA PCR Primer, Index 7 (100% over 47bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA360570.3083393756403427RNA PCR Primer, Index 1 (100% over 32bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT349130.29855652499462754RNA PCR Primer, Index 7 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC338800.28972288450771866No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT322790.2760320244694407RNA PCR Primer, Index 7 (100% over 38bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTAT310300.265351272322152RNA PCR Primer, Index 7 (100% over 48bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA305600.2613320941722515RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA297600.2544909398745486No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCG296550.2535930383729751RNA PCR Primer, Index 7 (100% over 45bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA290050.2480346005060915RNA PCR Primer, Index 1 (100% over 32bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC288090.2463585177031543RNA PCR Primer, Index 7 (100% over 44bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC286900.245340896001371RNA PCR Primer, Index 1 (100% over 27bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA279420.23894441673301875No Hit
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC270780.23155597009149959RNA PCR Primer, Index 7 (100% over 39bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA268660.2297430642026083RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATC265320.22688688228331733RNA PCR Primer, Index 7 (100% over 39bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTC262860.2247832273367737RNA PCR Primer, Index 7 (100% over 44bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA255450.21844660816852635No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA255120.2181644105537461RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA250740.21441887857575376RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA235280.20119834789544289RNA PCR Primer, Index 1 (100% over 32bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG230620.19721337551703091RNA PCR Primer, Index 7 (100% over 36bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG228970.1958023874431297RNA PCR Primer, Index 7 (100% over 36bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC227480.19452822245518253RNA PCR Primer, Index 1 (100% over 27bp)
TCGTACGACTCTTAGCGGTGGCATTCTCGGGTGCCAAGGAACTCCAGTCA225060.1924587732801274RNA PCR Primer, Index 1 (96% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC222840.19056035296251483RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC216400.18505322375286398RNA PCR Primer, Index 1 (100% over 33bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC210570.180067732558413RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA209610.17924679404268865No Hit
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA207260.1772372049677384RNA PCR Primer, Index 7 (100% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA199150.17030198479844205RNA PCR Primer, Index 7 (100% over 37bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC195830.16746290576489534RNA PCR Primer, Index 1 (100% over 24bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA195750.16739449422191832RNA PCR Primer, Index 7 (100% over 40bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC189420.16198143088386088No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT187130.16002315046614343RNA PCR Primer, Index 1 (100% over 30bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC185280.15844113353479963RNA PCR Primer, Index 1 (100% over 26bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA181930.15557640017263652RNA PCR Primer, Index 7 (100% over 40bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA180190.15408844911288616RNA PCR Primer, Index 7 (100% over 37bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA178280.15245512352430957RNA PCR Primer, Index 7 (100% over 37bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA175960.15047118877797572RNA PCR Primer, Index 7 (100% over 35bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAG173480.1483504309456878RNA PCR Primer, Index 7 (100% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC169050.14456214175333482RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA168350.14396354075228582No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC167780.1434761085085745RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA164340.14053441216056223RNA PCR Primer, Index 1 (100% over 32bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA161230.13787491342733021RNA PCR Primer, Index 7 (100% over 40bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA158260.135335134894308RNA PCR Primer, Index 1 (100% over 32bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGA154230.13188890341684018RNA PCR Primer, Index 7 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG152520.13042660668570616No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCATCTCGTATG146180.1250049919047766RNA PCR Primer, Index 7 (100% over 49bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT145050.12403867886022607RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC144770.12379923845980646RNA PCR Primer, Index 1 (100% over 26bp)
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCA144710.1237479298025737RNA PCR Primer, Index 7 (100% over 40bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA141380.12090029932615486RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG140060.11977150886703386RNA PCR Primer, Index 1 (100% over 29bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA139670.11943800259502084RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA139560.11934393672342744RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT138040.11804411740686388RNA PCR Primer, Index 1 (100% over 25bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGAT136500.11672719520455607RNA PCR Primer, Index 7 (100% over 38bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC131320.11229754779679343RNA PCR Primer, Index 1 (100% over 31bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA130620.11169894679574441RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA126730.10837243551848638RNA PCR Primer, Index 1 (100% over 28bp)
CAAACGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACT126410.10809878934657828RNA PCR Primer, Index 1 (100% over 25bp)
GGAATTCCCCGGTGCCCAGGAACTCCAGTCACCAGATCATCTCGTATGCC125490.10731205660234241RNA PCR Primer, Index 7 (97% over 40bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC122720.10494330692676279RNA PCR Primer, Index 1 (100% over 31bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAGATCAT122070.10438746314007444RNA PCR Primer, Index 7 (100% over 41bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG121540.1039342366678516RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)