FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002258455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16351155
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9275525.672700185399747RNA PCR Primer, Index 1 (100% over 32bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG6734284.118534745710624RNA PCR Primer, Index 15 (97% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3711772.2700353583584767RNA PCR Primer, Index 1 (100% over 33bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA2570421.5720112738213294No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG2475211.5137829712946884RNA PCR Primer, Index 15 (97% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2337781.4297338628372125RNA PCR Primer, Index 6 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1562500.9555899873739806RNA PCR Primer, Index 1 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1481570.9060950128599479No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1472670.9006519722918657No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCGTATGC1439310.8802497438254361RNA PCR Primer, Index 20 (100% over 50bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCA1413140.8642447582449069RNA PCR Primer, Index 20 (100% over 40bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1321240.8080407775475189RNA PCR Primer, Index 6 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1224360.7487911404423725RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1118110.6838110213009417No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT1054840.6451165070602046RNA PCR Primer, Index 1 (97% over 35bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1024660.6266590953360787RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC745690.4560472945183383No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA697330.42647140217311863No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG641150.39211297305908976RNA PCR Primer, Index 6 (100% over 34bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC637180.3896850100191699No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC542550.33181142249584206RNA PCR Primer, Index 20 (100% over 44bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA511090.31257119145405937RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA511050.31254672835038255RNA PCR Primer, Index 1 (100% over 32bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG477630.292107805228438RNA PCR Primer, Index 6 (100% over 34bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG424240.259455677595864Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA407690.24933406844959882RNA PCR Primer, Index 1 (100% over 32bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC382570.2339712393405848RNA PCR Primer, Index 1 (100% over 27bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA361580.22113422568619767RNA PCR Primer, Index 1 (100% over 32bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC350810.21454753502122634RNA PCR Primer, Index 20 (100% over 38bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA330890.20236490939019294RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA329590.20156985852069778No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA320660.196108470624858RNA PCR Primer, Index 1 (100% over 23bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC310180.18969913746154324RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA306700.1875708474416639RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA300880.1840114658566933No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA300660.18387691878647106RNA PCR Primer, Index 1 (100% over 28bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGC300460.18375460326808718RNA PCR Primer, Index 20 (100% over 38bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA284500.1739938249010544No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT282040.1724893440249328RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA280450.171516935653781No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA274860.1680982169149519RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA263420.16110176926339453RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA261560.15996423494242454RNA PCR Primer, Index 1 (100% over 32bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC261290.15979910899260633RNA PCR Primer, Index 20 (100% over 39bp)
CTCGTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCAT255870.15648435844440345RNA PCR Primer, Index 20 (100% over 41bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC255570.15630088516682766RNA PCR Primer, Index 1 (100% over 33bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG250680.15331027074234205RNA PCR Primer, Index 17 (97% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT250650.15329192341458447No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC246960.15103520210040208RNA PCR Primer, Index 1 (100% over 31bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT245050.14986708889983613RNA PCR Primer, Index 1 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC244250.14937782682630066No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA242330.1482035978498155No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG240860.14730457878969408Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC233790.14298072521482427No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG230340.14087078252270252RNA PCR Primer, Index 15 (97% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC229950.140632267261854RNA PCR Primer, Index 1 (100% over 26bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA227450.1391033232820556RNA PCR Primer, Index 1 (100% over 32bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCC227030.1388464606934495RNA PCR Primer, Index 20 (100% over 39bp)
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA225950.1381859568941766RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG221990.13576410963017596RNA PCR Primer, Index 15 (97% over 36bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC221810.1356540256636305RNA PCR Primer, Index 1 (100% over 26bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG219530.1342596287540544RNA PCR Primer, Index 17 (97% over 37bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTCG217400.13295696848326616RNA PCR Primer, Index 20 (100% over 45bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA216530.13242489597829632RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC213470.13055346854702313No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA211810.129538249744437RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC211150.12913460853377023RNA PCR Primer, Index 1 (100% over 24bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT200930.12288428554435452RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC193670.11844423222702005No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC190440.11646883660512056No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT190140.11628536332754474No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC187710.11479922977918072RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC183690.11234068785966496RNA PCR Primer, Index 1 (100% over 27bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG179080.10952131516091677No Hit
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGG172450.1054665557264915RNA PCR Primer, Index 17 (97% over 37bp)
NCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA171870.10511184072317828RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC170990.10457365244228925No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT170850.10448803157942055No Hit
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTGGCCATCTC169690.10377860157279409RNA PCR Primer, Index 20 (100% over 44bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC164620.100677903181763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)