FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258459

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258459
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13982161
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG4163872.9779874512959763RNA PCR Primer, Index 10 (97% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3697292.6442908217120373RNA PCR Primer, Index 1 (100% over 33bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3309522.366958869948644RNA PCR Primer, Index 1 (100% over 32bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG1894671.3550623540953362RNA PCR Primer, Index 10 (97% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1630051.1658069163986884RNA PCR Primer, Index 3 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC1256800.8988596254899367RNA PCR Primer, Index 5 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1226450.8771533956732439RNA PCR Primer, Index 3 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT1067360.7633727004001741No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA987420.7061998499373594No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAA961820.6878908060063105RNA PCR Primer, Index 48 (100% over 40bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA904340.646781280804877No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC901570.6448001850357752RNA PCR Primer, Index 5 (97% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC900350.6439276446609361RNA PCR Primer, Index 5 (97% over 35bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA757940.5420764358241905No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC651460.4659222562234836No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC641550.4588346536704877RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA622050.444888311613634RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC618500.44234936216225806No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC617990.4419846116776942No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT610060.4363130992412403RNA PCR Primer, Index 3 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC539920.38614917965828033RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG502950.3597083455125427Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT496490.3550881727080671RNA PCR Primer, Index 3 (100% over 34bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA490790.35101154964529446RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC475190.33985447599981145RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC461090.3297701978971634RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG460890.3296271584914521No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG460190.32912652057146247No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA453280.3241845091041363RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC442380.3163888614928694RNA PCR Primer, Index 1 (100% over 24bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG433390.3099592402061455Illumina Small RNA Adapter 2 (100% over 21bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA412400.2949472545767425No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGC411050.29398173858819104RNA PCR Primer, Index 48 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC403910.2888752318042969No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA400900.28672248874834155No Hit
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT387630.27723182417939546No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTCGTATGCC379470.27139581642637356RNA PCR Primer, Index 48 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT377280.26982953493383466No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC343560.245713091130906No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA336520.24067810404986753RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA336300.24052076070358508No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA333090.2382249782419184No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA326150.2332615108637356RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA317190.22685334548786842RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC313730.2243787637690626RNA PCR Primer, Index 1 (100% over 31bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA296880.21232769383788386RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC281410.20126359580611322No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC281170.20109194851925966RNA PCR Primer, Index 1 (100% over 33bp)
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT280300.20046972710441538No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG279770.2000906726792804No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG272310.19475530284624815No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT271430.19412592946111834No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC271130.19391137035255138RNA PCR Primer, Index 1 (100% over 26bp)
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC270830.19369681124398438No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG269700.19288863860171543Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT267990.19166565168288363No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG260910.1866020567207029Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGC253780.18150270190709433RNA PCR Primer, Index 48 (100% over 38bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC252160.18034408272083266RNA PCR Primer, Index 1 (100% over 24bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCAATCTC246920.17659645029119603RNA PCR Primer, Index 48 (100% over 44bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG238700.17071753071646079RNA PCR Primer, Index 10 (97% over 36bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC234910.16800693397823127RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGC232640.16638343672340777RNA PCR Primer, Index 48 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT227670.16282890749148146No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT217140.15529788278078044No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC215780.15432521482194347No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT215350.15401768009966413RNA PCR Primer, Index 1 (100% over 30bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC212660.15209380009284687RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC206130.14742356349637226RNA PCR Primer, Index 5 (97% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT199390.14260313552390078RNA PCR Primer, Index 1 (100% over 25bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG198330.14184502667363078RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG195310.13968513164738985No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA193300.13824758561999106RNA PCR Primer, Index 1 (100% over 22bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTC191220.13675997580059335RNA PCR Primer, Index 5 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG189210.13532242977319459No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA187120.13382766798351126RNA PCR Primer, Index 1 (100% over 32bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGG187040.13377045222122674RNA PCR Primer, Index 48 (100% over 37bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGGCA185940.1329837354898145RNA PCR Primer, Index 48 (100% over 39bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA184870.13221847466925893RNA PCR Primer, Index 1 (100% over 28bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA181410.12974389295045308RNA PCR Primer, Index 1 (100% over 28bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA178500.12766266959735337RNA PCR Primer, Index 1 (100% over 23bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCG178300.12751963019164206RNA PCR Primer, Index 10 (97% over 36bp)
CAAACGAGAACTTTGAAGGCCGAATGGAATTCTCGGGTGCCAAGGAACTC176930.1265398102625195RNA PCR Primer, Index 1 (100% over 26bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG176920.12653265829223392RNA PCR Primer, Index 1 (100% over 29bp)
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAA176070.12592474081796082RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG173610.12416535612771158No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA171700.12279932980316847RNA PCR Primer, Index 1 (100% over 23bp)
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTTGGAATTCT164360.11754978361356302No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT161590.11556868784446124No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT158950.1136805676890718No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC155410.11114877020798145RNA PCR Primer, Index 1 (100% over 27bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTCGG154720.11065528425827739RNA PCR Primer, Index 48 (100% over 37bp)
TCGCTGGTTCGATTCCGGCCCGAAGGACCTGGAATTCTCGGGTGCCAAGG153530.10980419979429504Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA153320.10965400841829813RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA152530.10908900276573844No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT152430.10901748306288277No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG152320.10893881138974155No Hit
CCCCGGGTTCAATCCCCGGCACCTCCACCATGGAATTCTCGGGTGCCAAG151540.10838095770746739No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC150510.1076443047680541No Hit
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG148730.10637125405722335RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC148030.10587061613723371No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT144190.10312425954757637No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA142590.10197994430188582No Hit
CTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTG141700.10134341894647042No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA140990.10083562905619525RNA PCR Primer, Index 1 (100% over 22bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT140370.10039220689849015RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)