FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258460

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258460
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9208137
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1871822.032789042995342RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1543031.6757244163504517No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG963781.0466612301706633RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA937411.0180235155059054No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG784910.8524091246687576Illumina Small RNA Adapter 2 (100% over 21bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA782180.8494443555737714No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG759580.8249008458497088No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC719870.7817759444717209No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC675900.734024700110348RNA PCR Primer, Index 1 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC642990.6982845715696888No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA578400.6281400895751226No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA440040.47788168225559635RNA PCR Primer, Index 1 (100% over 28bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC401200.43570159740238446RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG396100.43016301777438803Illumina Small RNA Adapter 2 (100% over 21bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG375020.4072702219786695RNA PCR Primer, Index 18 (100% over 38bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA362860.39406451055191727RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC356990.387689713999694No Hit
GTTTCCGTAGTGTAGTGGTTATCTGGAATTCTCGGGTGCCAAGGAACTCC342830.3723120105619627RNA PCR Primer, Index 1 (100% over 27bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC333870.3625814863527769RNA PCR Primer, Index 1 (97% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC323950.35180840597832114RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCATCTCGTATGC300620.32647211917025126RNA PCR Primer, Index 18 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT299760.3255381626055303RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG298620.32430012715927226No Hit
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT289870.3147976621112392RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG285250.30978036056587777No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT273560.2970850672617056RNA PCR Primer, Index 1 (100% over 30bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG273090.2965746491391255RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT266250.2891464364615774No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT262400.28496535184044286RNA PCR Primer, Index 1 (97% over 35bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT258130.2803281488970027RNA PCR Primer, Index 1 (97% over 35bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT253980.27582126547422137RNA PCR Primer, Index 1 (97% over 35bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG248580.2699568870445781No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG237030.25741363318117444No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA235620.25588237881343423No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA233760.2538624262432238RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC228260.24788944821303158No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG227610.2471835508094634Illumina Small RNA Adapter 2 (100% over 21bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC223390.24260064766629774RNA PCR Primer, Index 1 (100% over 26bp)
GTCAGGATGGCCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG215280.23379322006177797RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC211660.229861914521906No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGGAATTCTCGGGTGCCAAGGAAC208350.2262672677437358RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC204440.22202102336227186RNA PCR Primer, Index 1 (100% over 26bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG202680.22010967039261037No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC195730.2125619981544584No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC195470.21227963919303114RNA PCR Primer, Index 1 (97% over 36bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA192680.20924971033771544No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT184440.20030110325248204RNA PCR Primer, Index 1 (100% over 30bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA184040.19986670485028624RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC183080.1988241486850163RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA180270.19577249990959086No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC178530.1938828668600391RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT176510.19168915492895033No Hit
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC171760.18653067390287525RNA PCR Primer, Index 1 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG170850.1855424175378798No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA168990.18352246496766936RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT167290.18167627175833723RNA PCR Primer, Index 1 (100% over 30bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA167020.18138305283685505RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA164150.17826624430110022No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC163660.17773410625841038RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC160910.17474761724331425RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCTGGAATTCTCGGGTGCCAAGGAAC160180.17395484015930693RNA PCR Primer, Index 1 (100% over 24bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG159360.17306432343480554RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG158990.17266250491277443No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA157630.17118555034530872RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC156000.16941537685636085No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC155400.16876377925306715No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT152590.16571213047764166No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC151740.1647890338729756RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG150870.16384421734819976RNA PCR Primer, Index 6 (100% over 34bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC148200.1609446080135428RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC147950.16067310901217044No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA147140.15979345224772395RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC146440.1590332550438813RNA PCR Primer, Index 1 (100% over 27bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG144350.15676352339240826No Hit
GTAGTCGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCC144250.1566549237918593RNA PCR Primer, Index 18 (100% over 37bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC141260.15340779573544572No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA140410.15248469913077967RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA139830.15185482144759577No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC134200.1457406639366899RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA129750.14090798171226165No Hit
CGCGACCTCAGATCAGACTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA129030.14012606458830923RNA PCR Primer, Index 1 (100% over 32bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA128580.13963736638583896RNA PCR Primer, Index 1 (100% over 28bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTC127400.13835589109936136RNA PCR Primer, Index 1 (97% over 36bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA127240.13818213173848304RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC123620.1342508261986111RNA PCR Primer, Index 1 (100% over 31bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA123390.1340010471173485RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCTGGAATTCTCGGGTGCCAAGGA119710.13000458181714716RNA PCR Primer, Index 1 (100% over 22bp)
GGGGATGTAGCTCAGTGGTAGATGGAATTCTCGGGTGCCAAGGAACTCCA118360.12853848720973635RNA PCR Primer, Index 1 (100% over 28bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG117620.12773485016567412RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA116850.1268986332414472RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG115070.1249655603516759RNA PCR Primer, Index 1 (100% over 29bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT113610.12338000618366127RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCTGGAATTCTCGGGTGCCAAGGAA112630.12231573009828156RNA PCR Primer, Index 1 (100% over 23bp)
GTTTCCGTAGTGTAGTGGTCATCTGGAATTCTCGGGTGCCAAGGAACTCC112550.1222288504178424RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCTGGAATTCTCGGGTGCCAAGGA111910.12153381297432912RNA PCR Primer, Index 1 (100% over 22bp)
CGACTCTTAGCGGTGGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC111410.12099081497158437RNA PCR Primer, Index 1 (100% over 31bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGC108740.11809120563692742RNA PCR Primer, Index 18 (100% over 39bp)
GTCTACGGCCATACCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC106730.11590835366589354RNA PCR Primer, Index 1 (100% over 31bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC104670.11367120189458518No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG104290.11325852341249919Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC103820.11274810528991912RNA PCR Primer, Index 1 (100% over 24bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG103520.11242230648827228RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCGCA102960.11181414872519817RNA PCR Primer, Index 18 (100% over 40bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA100460.10909915871147444RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGTGGAATTCTCGGGTGCCAAGGAACTC98730.10722038562197761RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGTGGAATTCTCGGGTGCCAAGGAACT98060.10649276829829964RNA PCR Primer, Index 1 (100% over 25bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTCTGGAATTCTCGGGTGC96990.10533075257242588No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA96500.10479861452973604RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGTGGAATTCTCGGGTGCCAAGG95600.1038212181247955Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA94310.10242028327771405RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC93910.10198588487551824No Hit
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTCCG93520.10156234643337735RNA PCR Primer, Index 18 (100% over 38bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG93390.10142116695266372RNA PCR Primer, Index 6 (100% over 34bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA93180.10119310779151093RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG92630.10059580998849169No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)