FastQCFastQC Report
Wed 1 May 2024
EGAF00002258463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55437478
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGC1182780.213353861443697No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1181910.21319692789776623No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG1151400.20769343078702102TruSeq Adapter, Index 22 (97% over 39bp)
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG1051070.18959556565686483No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1003180.18095700529522646TruSeq Adapter, Index 22 (97% over 40bp)
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC913140.16471528520832063No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC789500.14241268334753612No Hit
CCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGG741810.13381019966312319No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA687200.12395946294670908No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG681280.12289159330083521No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC604240.10899485723358483No Hit
GGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCGGCC575750.10385573456281687No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)