FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763113
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1623131.8522299096222998RNA PCR Primer, Index 1 (100% over 33bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1499931.711640600777372RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1179191.3456291160458618RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1142841.304148423054684No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT1038481.1850583234519514RNA PCR Primer, Index 9 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA832670.9501988619797553No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT793280.9052490821469493RNA PCR Primer, Index 9 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC791210.9028869078830776No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT706600.8063344612810539RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT686120.7829637709795594No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC656930.7496536904180056No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC654510.7468921147085517No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT552920.6309629922608553RNA PCR Primer, Index 1 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA516480.5893795960408134No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA460220.5251786665309462No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG430810.49161753363216926No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT411410.4694792820770427No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG394650.45035365856859316No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGC374630.4275078958812924RNA PCR Primer, Index 21 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG365120.4166555880313309No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG351630.40126151517160624RNA PCR Primer, Index 6 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC343530.3920182245738473No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG342990.3914020052006633No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG341630.3898500452978297Illumina Small RNA Adapter 2 (100% over 21bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA332140.37902056038761567RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG324920.370781479138749RNA PCR Primer, Index 6 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC318920.363934597214483RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG312350.3564372615074118RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG300630.3430630188153456No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC295230.3369008250835063No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA273510.3121151125176635RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT267620.30539375676200914RNA PCR Primer, Index 1 (97% over 35bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCGTATGCC263560.30076069999325583RNA PCR Primer, Index 21 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC261080.2979306554645592No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA260130.29684656582655045RNA PCR Primer, Index 1 (100% over 28bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC259800.29646998732071583RNA PCR Primer, Index 1 (100% over 26bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC243100.27741283263150895RNA PCR Primer, Index 1 (100% over 27bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG241580.27567828921069487RNA PCR Primer, Index 21 (100% over 39bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTC239330.27311070848909513RNA PCR Primer, Index 21 (100% over 44bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC237790.2713533421285335RNA PCR Primer, Index 1 (100% over 27bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC235770.26904822521403066RNA PCR Primer, Index 21 (100% over 38bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA230260.26276050531357975No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG229300.2616650042056972Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC226970.2590061317251073RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC223890.2554913990039841RNA PCR Primer, Index 1 (100% over 24bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA219570.2505616440185126RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT216700.24728655216473872RNA PCR Primer, Index 1 (100% over 30bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT209590.23917299708448356RNA PCR Primer, Index 1 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT208930.2384198400728143No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA208910.23839701713306677No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA207350.2366168278327576RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC204250.23307927217188687No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT202600.23119637964271372RNA PCR Primer, Index 9 (97% over 36bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG199660.2278414074998234No Hit
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT197050.22486301386276772RNA PCR Primer, Index 1 (100% over 30bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGA195930.2235849292369047RNA PCR Primer, Index 21 (100% over 40bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT194980.22250083959889597RNA PCR Primer, Index 9 (97% over 36bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT192280.2194197427329763RNA PCR Primer, Index 1 (100% over 30bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA188630.21525455622904782RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC184580.21063291093016834No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC180900.2064334900166185RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA180190.2056232756555804RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA179410.20473318100542579RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC177570.20263347054865094No Hit
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG175470.20023706187515786RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC174420.19903885753841127RNA PCR Primer, Index 21 (100% over 38bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG170350.19439438929978423RNA PCR Primer, Index 1 (100% over 29bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC167790.1914730530120974No Hit
CGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTTGGAATTCT160780.18347361263058No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT158480.1808489745596114RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC158370.18072344839099988RNA PCR Primer, Index 1 (100% over 26bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA154810.1766609651159354No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT154800.17664955364606164No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC147650.16849035268631135No Hit
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCG147460.16827353475870963RNA PCR Primer, Index 21 (100% over 39bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG146150.16677863220524486No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG145340.16585430314546898Illumina Small RNA Adapter 2 (100% over 21bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA141880.16190593456914226RNA PCR Primer, Index 1 (100% over 22bp)
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC139980.1597377552931247RNA PCR Primer, Index 21 (100% over 38bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGA139970.15972634382325093RNA PCR Primer, Index 21 (100% over 40bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC138190.15769510218571872RNA PCR Primer, Index 1 (100% over 24bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT136830.1561431422828851RNA PCR Primer, Index 21 (100% over 37bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA135250.15434013004282837RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA132870.15162420021286954RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA131900.1505172876351132RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT130980.1494674324067258No Hit
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT129350.1476073628173002RNA PCR Primer, Index 9 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG123060.14042954826669474No Hit
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTC120640.13766797255724078RNA PCR Primer, Index 21 (100% over 38bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT119560.13643553381087292RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC118160.1348379280285442No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC117890.1345298183419522No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA115260.13152860176514897RNA PCR Primer, Index 1 (100% over 28bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC114310.1304445121271402RNA PCR Primer, Index 1 (100% over 26bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA113930.1300108762719367RNA PCR Primer, Index 1 (100% over 28bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT113630.1296685321757234RNA PCR Primer, Index 9 (97% over 36bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA113580.12961147482635452RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA110270.12583427829813446No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG107870.12309552552842809No Hit
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT107310.12245648321549658RNA PCR Primer, Index 21 (100% over 37bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG106530.12156638856534202No Hit
GTAGTCGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTT106110.12108710683064339RNA PCR Primer, Index 21 (100% over 37bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG105020.11984325661440175No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA103350.11793754114548106No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT102130.11654534182088032No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG101940.11632852389327857RNA PCR Primer, Index 1 (100% over 29bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT101590.11592912244769639No Hit
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC101360.11566665864059951No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG100870.11510749661678447RNA PCR Primer, Index 6 (100% over 34bp)
TCTGACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGAT99840.11393211521978548RNA PCR Primer, Index 21 (100% over 41bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT99800.11388646934029037RNA PCR Primer, Index 1 (97% over 35bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA98290.11216333738935011RNA PCR Primer, Index 1 (100% over 28bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGATCTCG97810.11161558683540883RNA PCR Primer, Index 21 (100% over 45bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG97600.11137594596805953RNA PCR Primer, Index 1 (100% over 29bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCG96910.11058855454676896No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG96870.11054290866727384No Hit
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC96500.11012068428194409RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC95790.10931046992090597RNA PCR Primer, Index 1 (100% over 27bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTT94730.10810085411428565RNA PCR Primer, Index 9 (97% over 36bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG94450.10778133295781989RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA92240.10525939811571527RNA PCR Primer, Index 1 (100% over 23bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC91560.10448341816429846No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGT90300.10304557296020261RNA PCR Primer, Index 1 (97% over 35bp)
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTTTCGA88240.10069481016620463RNA PCR Primer, Index 21 (100% over 40bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG88070.10050081517835044Illumina Small RNA Adapter 2 (100% over 21bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG88000.100420934889234No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA87870.1002725857808749RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)