FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258473

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258473
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9766848
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3345763.4256292306381755RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1849691.8938453838945788RNA PCR Primer, Index 6 (100% over 34bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT1477591.5128626963376517RNA PCR Primer, Index 9 (100% over 36bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1416221.4500276854928018RNA PCR Primer, Index 1 (100% over 33bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG718390.7355392445955953RNA PCR Primer, Index 6 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC670080.6860759991350331RNA PCR Primer, Index 9 (100% over 50bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT639210.6544690774341938RNA PCR Primer, Index 9 (100% over 36bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA609170.6237119693067815RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA574830.5885522125459514No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA542610.5555630639485738RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG477630.48903187599520337No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC470650.4818852510042135No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC456040.4669264843683448RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG443640.45423047435569797Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA442380.452940395918929RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC442200.4527560989993906No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA420990.4310397786471132RNA PCR Primer, Index 7 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC393200.40258638201393115No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA389980.3992895148977439No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC386480.3957059636844968RNA PCR Primer, Index 1 (100% over 27bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC382680.3918152509386857RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA369330.378146562739586No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA364260.37295553283925376RNA PCR Primer, Index 1 (100% over 23bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA344260.35247809733498464RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC339380.347481603071943No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT323370.3310894159507755No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC314440.32194624099811936No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA304670.3119430137542839RNA PCR Primer, Index 1 (100% over 23bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA296360.30343463930226006RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG292260.2992367650238849No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA289720.29663613071484274RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC265170.27150007863335235RNA PCR Primer, Index 1 (100% over 33bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG263200.2694830512361818RNA PCR Primer, Index 6 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA262770.26904278637284007RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC260540.266759552314114RNA PCR Primer, Index 1 (100% over 26bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG260540.266759552314114RNA PCR Primer, Index 6 (100% over 34bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA248010.2539304389706894RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC241560.2473264660205626RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT240170.2459032842530159RNA PCR Primer, Index 1 (100% over 25bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC235120.24073273178818796RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA233060.23862355593124823RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG232990.23855188490698329No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC225540.23092404018164306RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC221510.2267978369275328RNA PCR Primer, Index 9 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG217690.2228866467462174No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA213430.21852495298380809RNA PCR Primer, Index 1 (100% over 32bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA212020.2170812937807571RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA209920.21493116305280885No Hit
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA204140.2090131841920751RNA PCR Primer, Index 1 (100% over 22bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA203570.2084295772802034RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC199670.2044364773568709RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT197350.2020610948383757No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT194080.1987130341334277RNA PCR Primer, Index 1 (100% over 30bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG191250.1958154770095736RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC191050.19561070265453093No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA190930.1954878380415053RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA189880.19441277267753118RNA PCR Primer, Index 1 (100% over 32bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC188430.19292815860347165RNA PCR Primer, Index 1 (100% over 31bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG187200.19166879631995912No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA184740.18915007175293402No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA183640.1880238128001992RNA PCR Primer, Index 1 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT179370.18365188032003774No Hit
TCTCCCAACCCTTGTACCAGTGTGGAATTCTCGGGTGCCAAGGAACTCCA177260.18149151087433735RNA PCR Primer, Index 1 (100% over 28bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAATGGAATTCTCGGGTGCCAA165340.16928695931379295No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT161430.16528362067270833RNA PCR Primer, Index 1 (100% over 25bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA160480.16431094248625555RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG159250.163051580202743No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA155010.15871036387583795RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG153280.15693906570471866No Hit
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA152330.1559663875182659RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC151080.15468654779924906No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT145400.14887095611603662No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG144930.1483897363816863RNA PCR Primer, Index 1 (100% over 29bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC144200.14764230998578046RNA PCR Primer, Index 1 (100% over 31bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC143070.14648533487978926No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA140270.1436184939091916RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA138090.14138645343922626RNA PCR Primer, Index 1 (100% over 28bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC137540.14082332396285885RNA PCR Primer, Index 1 (100% over 27bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT136950.1402192396154829No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA132120.1352739389412019RNA PCR Primer, Index 1 (100% over 22bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA131200.13433197690800552RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG130570.13368693768962106RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC129960.13306237590674086RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG127570.13061532236398068No Hit
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC124700.12767681036911807RNA PCR Primer, Index 1 (100% over 24bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA123040.12597718322226373RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA121890.12479973068076824No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC120520.1233970263487258RNA PCR Primer, Index 1 (100% over 31bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA120170.1230386712274011RNA PCR Primer, Index 1 (100% over 22bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC117490.12029469486982904No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT116660.11944488129640186No Hit
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG115230.11798074465784662No Hit
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC113230.11593300110741972RNA PCR Primer, Index 1 (100% over 26bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA110900.11354737987117236No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC109530.11214467553912993RNA PCR Primer, Index 1 (100% over 31bp)
GTCGGTGCTGAAATCCAGAGGCTGTTTCTGAGCTGGAATTCTCGGGTGCC109420.11203204964385645No Hit
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT108920.1115201137562497RNA PCR Primer, Index 9 (100% over 36bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG108080.11066006146507042No Hit
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC107970.11054743556979693RNA PCR Primer, Index 1 (100% over 33bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA105810.10833587253533587RNA PCR Primer, Index 1 (100% over 32bp)
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA105400.10791608510749835RNA PCR Primer, Index 1 (100% over 23bp)
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA104490.10698436179205409No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG102660.10511067644341347No Hit
TACCCTGTAGAACCGAATTTGTGTGGAATTCTCGGGTGCCAAGGAACTCC101620.10404584979719148RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA100200.10259195187638838No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC99450.10182404804497827No Hit
TCATATCATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCT98920.10128139600411513No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT98420.10076946011650842RNA PCR Primer, Index 1 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)