FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002258474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16679695
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA3859232.3137293577610385No Hit
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA3313211.986373252028889RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT3040311.8227611476109125No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC2212351.3263731740898141No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2206341.3227699906982713No Hit
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT2086441.2508861822713184RNA PCR Primer, Index 3 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1660370.9954438615334392RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1605620.9626195203209651RNA PCR Primer, Index 3 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG1541200.9239977109893197No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1497610.8978641396020731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1353920.8117174804455358No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG1311460.7862613794796608No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA1220810.731913862933345RNA PCR Primer, Index 3 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC1216980.7296176578768376No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1082810.6491785371375196No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1027650.6161083880730434No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1008370.6045494237154816No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG978920.5868932255655754No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT917340.5499740852575542No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG913550.5477018614549007No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG892040.5348059421949862Illumina Small RNA Adapter 2 (100% over 21bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA879280.527155922215604RNA PCR Primer, Index 1 (100% over 28bp)
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC878840.5268921284232115No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT785920.47118367572068914No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC782210.4689594144257434No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT730060.43769385471377026RNA PCR Primer, Index 3 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC684430.4103372393799766No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA671360.4025013646832271RNA PCR Primer, Index 7 (97% over 35bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA633400.3797431547759117RNA PCR Primer, Index 7 (97% over 35bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGC629350.3773150528232081RNA PCR Primer, Index 42 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA616110.36937725779757963RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT585140.35080977200122665RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA550500.33004200616378176No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG533790.3200238373663308No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT509860.30567705224825753RNA PCR Primer, Index 3 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC463960.2781585634509504No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA445830.26728906014168724RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT443360.2658082177162112No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT424910.25474686437611715No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC418990.2511976388057455RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT416710.2498307073360754RNA PCR Primer, Index 3 (100% over 34bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG413420.24785824920659522No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA396210.2375403147359709No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA390530.23413497668872243RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA386840.2319227060207036RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC378120.2266947926805616No Hit
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC371920.22297769833321293RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTCGTATGCC353820.2121261809643402RNA PCR Primer, Index 42 (100% over 50bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC335800.20132262610317517RNA PCR Primer, Index 1 (100% over 27bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA332700.1994640789295008No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC329030.19726379888840895RNA PCR Primer, Index 1 (100% over 33bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC323090.19370258269111035RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT311760.18690989253700382No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG302860.18157406355451944No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTTGGA296910.1780068520437574No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA296040.1774852597724359RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA286850.17197556669951097RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTGGAA282650.16945753504485545No Hit
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCT277980.166657723657417No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGA276140.16555458598013933RNA PCR Primer, Index 42 (100% over 40bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA275140.16495505463379276RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATGGAAT274510.16457734988559444No Hit
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC268290.16084826491131882RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATTGGAATT265460.15915159120115804No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC264850.15878587707988664RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA257300.15425941541497012No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC251370.150704194531135RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA251350.15069220390420807RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC249730.14972096312312666RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC249610.14964901936156508RNA PCR Primer, Index 1 (100% over 26bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA248700.1491034458363897RNA PCR Primer, Index 1 (100% over 23bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT241140.1445709888580097RNA PCR Primer, Index 1 (100% over 25bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATC233320.1398826537295796RNA PCR Primer, Index 42 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA229170.13739459864224138No Hit
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG228800.13717277204409314RNA PCR Primer, Index 1 (100% over 29bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG228630.13707085171521421Illumina Small RNA Adapter 2 (100% over 21bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT226170.13559600460320168RNA PCR Primer, Index 1 (100% over 30bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA221440.13276022133498244RNA PCR Primer, Index 1 (100% over 32bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA217630.13047600690540204RNA PCR Primer, Index 7 (97% over 35bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC216890.1300323537091056RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAA211670.12690280008117655RNA PCR Primer, Index 3 (97% over 36bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG195230.11704650474723909No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAATCGATCTC194900.11684865940294473RNA PCR Primer, Index 42 (100% over 44bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC185740.11135695227041023RNA PCR Primer, Index 1 (100% over 26bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC185570.11125503194153132RNA PCR Primer, Index 1 (100% over 31bp)
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCTGGAATTCTC184200.11043367399703652No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG183680.1101219176969363No Hit
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT183540.11003798330844777No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC182000.10911470503507409RNA PCR Primer, Index 1 (100% over 33bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA173840.10422252924888614RNA PCR Primer, Index 1 (100% over 28bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG173470.10400070265073791RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG172220.10325128846780471RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTTGGAATTCTCGGG167770.10058337397656253No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)