FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002258479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17037144
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5552693.259167146794087RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC3388171.9886959927086372RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC3232531.8973426532052557RNA PCR Primer, Index 1 (100% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC2418501.4195454355495263RNA PCR Primer, Index 1 (100% over 36bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA2377741.395621237925793RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA2025171.188679276291848No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1491780.8756045027265134No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1473660.8649689173255799No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1402450.8231720058244505No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1314960.7718195021419083RNA PCR Primer, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1114650.6542469794233118No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1086800.6379003429213252No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1054750.6190885045052152RNA PCR Primer, Index 1 (100% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1052150.6175624271298052No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1031970.6057177188852779No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA947920.5563843329609705RNA PCR Primer, Index 1 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT907340.5325657868478426No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC885350.5196586939688953RNA PCR Primer, Index 1 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT867280.5090524562097967RNA PCR Primer, Index 1 (100% over 35bp)
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC818930.4806732865555401RNA PCR Primer, Index 1 (100% over 44bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC802180.4708418265408803No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC800070.46960335605545156No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC758100.4449689454993161RNA PCR Primer, Index 1 (100% over 27bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA747710.43887050552604356RNA PCR Primer, Index 1 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC733560.4305651228867937RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG731880.42957904212114423No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA701880.4119704570202612RNA PCR Primer, Index 1 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA688970.40439289589851446RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC687700.4036474657959104No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG681390.399943793396358Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG673240.3951601277772847No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT650450.3817834726289805RNA PCR Primer, Index 1 (100% over 25bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC622520.36538987990005833RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG615980.36155120834806587No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA601940.35331039052085256No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC573910.33685810250826076RNA PCR Primer, Index 1 (100% over 26bp)
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT552980.32457317963621135No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC546810.32095168063379637No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA538540.3160975806743196RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC519670.3050217806458641No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA496430.29138099672104667RNA PCR Primer, Index 1 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT496130.29120491087003786No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC432630.2539334057398353RNA PCR Primer, Index 1 (100% over 24bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG425290.24962517191848585RNA PCR Primer, Index 1 (100% over 39bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC418160.24544019819284263RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT417670.24515259130286157No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA408520.23978197284709224RNA PCR Primer, Index 1 (100% over 23bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA407600.2392419762373318RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA400250.23492787288761546RNA PCR Primer, Index 1 (100% over 32bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC395670.23223962889554728RNA PCR Primer, Index 1 (100% over 38bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC382620.22457989437666315RNA PCR Primer, Index 1 (100% over 33bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA380200.22315946851185856RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC343510.2016241689334785RNA PCR Primer, Index 1 (100% over 31bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA330150.19378247903521856RNA PCR Primer, Index 1 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC325140.1908418453233711No Hit
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG321320.18859968548719197RNA PCR Primer, Index 1 (100% over 45bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA302220.1773888863062964RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT300760.1765319351647201RNA PCR Primer, Index 1 (100% over 30bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT300540.17640280554064694No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC296910.1742721667434401No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT296810.17421347145977048RNA PCR Primer, Index 1 (100% over 30bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG284920.1672346022314538RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC284260.1668472133592344RNA PCR Primer, Index 1 (100% over 38bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA272850.16015008149253185RNA PCR Primer, Index 1 (100% over 28bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA271290.15923443506728593RNA PCR Primer, Index 1 (100% over 32bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG268020.15731509929128967RNA PCR Primer, Index 1 (100% over 39bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG263260.15452120378861622RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC261170.15329447235992136RNA PCR Primer, Index 1 (100% over 24bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA258940.15198556753408904RNA PCR Primer, Index 1 (100% over 37bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA252200.14802950541475732RNA PCR Primer, Index 1 (100% over 37bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC245740.1442377900897005RNA PCR Primer, Index 1 (100% over 38bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA244140.14329866555098672RNA PCR Primer, Index 1 (100% over 32bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC240070.14090976750563358RNA PCR Primer, Index 1 (100% over 31bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT239380.14050477004831327No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC239000.14028172797036875RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA238470.1399706429669198RNA PCR Primer, Index 1 (100% over 32bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA231010.13559197480516688No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTGGAATTCTCGGG230310.1351811078194796No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG229520.13471741507848967Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC219170.128642453218685RNA PCR Primer, Index 1 (100% over 36bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC218730.12838419397053874RNA PCR Primer, Index 1 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA216370.12699898527593592No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA214950.12616551224782746RNA PCR Primer, Index 1 (100% over 22bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG213010.12502682374463703Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA212830.12492117223403172RNA PCR Primer, Index 1 (100% over 28bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT211400.12408182967755628No Hit
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG209770.12312509655374164RNA PCR Primer, Index 1 (100% over 29bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA208290.12225640635543142RNA PCR Primer, Index 1 (100% over 32bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA204420.11998489887741748No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA202400.11879925414729135No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC201790.11844121291690674No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG201270.11813599744182476No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT197000.1156297088291324No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA194560.1141975439075939RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC194220.11399797994311722No Hit
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT192410.11293559530869728RNA PCR Primer, Index 1 (100% over 35bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC191600.11246016351097343RNA PCR Primer, Index 1 (100% over 44bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA191440.11236625105710207RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA189890.1114564741602231RNA PCR Primer, Index 1 (100% over 22bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA184230.10813432110452315RNA PCR Primer, Index 1 (100% over 37bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA184030.10801693053718393No Hit
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA179050.10509390541043734RNA PCR Primer, Index 1 (100% over 22bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT177750.1043308667227324RNA PCR Primer, Index 1 (100% over 35bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC172270.10111436517763776RNA PCR Primer, Index 1 (100% over 36bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTTGGAATTCTCGGGTG172190.10106740895070207No Hit
TCTGACACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT171540.10068588960684959RNA PCR Primer, Index 1 (100% over 41bp)
ATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGG170820.1002632835644284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)