FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16218224
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA9532915.877900070932551RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC5839113.6003387300607024RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGC4836182.9819417958464505RNA PCR Primer, Index 34 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA4429472.731168344943318No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC2670761.6467647752306296No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC2177971.34291522918909No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1827881.127053122462731No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1692971.0438689217758985No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1429010.8811137397041747No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1410420.8696513255705434No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1345820.8298195906037553No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG1229780.7582704493414323Illumina Small RNA Adapter 2 (100% over 21bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT1180720.7280205280183577RNA PCR Primer, Index 9 (97% over 36bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA951040.5864020622726631RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC898110.5537659363935287RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT759140.4680783789889694RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT747700.4610245856759655No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC744320.45894051037894157RNA PCR Primer, Index 2 (100% over 34bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA728490.44917988554110483RNA PCR Primer, Index 1 (100% over 28bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC720340.4441546743959141No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG603320.3720012746155189No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG558690.34448284843025967No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC525530.3240367132677413RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCA485820.29955191147933335RNA PCR Primer, Index 34 (100% over 40bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT477020.2941259166231765RNA PCR Primer, Index 9 (97% over 36bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCGTATGCC470230.2899392683193918RNA PCR Primer, Index 34 (100% over 50bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA465820.2872201049880677RNA PCR Primer, Index 7 (100% over 35bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG453420.2795743849634831No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT452160.2787974811545333No Hit
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA420890.2595167017054395RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC408730.25201896335875RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA401870.2477891537322459No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA392310.24189455022942094RNA PCR Primer, Index 7 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT389480.24014959961090684RNA PCR Primer, Index 1 (100% over 25bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC382760.23600611262984159RNA PCR Primer, Index 2 (100% over 34bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG382360.23575947650001627No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC374050.23063561090289542RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC361020.22260143897383586RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC355980.21949382373803694RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA338310.20859867270300375RNA PCR Primer, Index 1 (100% over 28bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA332420.204966955691326RNA PCR Primer, Index 7 (100% over 35bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC327980.20222929465026504RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG321950.19851125499314842No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT305930.18863347799364466No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA305240.18820803066969602RNA PCR Primer, Index 7 (100% over 35bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC303710.1872646474731142RNA PCR Primer, Index 1 (100% over 24bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA290840.17932912999598477RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG280710.1730830700081587No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA275260.16972265273928883No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA265030.16341493371900645RNA PCR Primer, Index 1 (100% over 23bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC263670.1625763708776004RNA PCR Primer, Index 2 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA262980.16215092355365174RNA PCR Primer, Index 1 (100% over 23bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGC261830.16144184468040398RNA PCR Primer, Index 34 (100% over 39bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA260360.16053545690329596RNA PCR Primer, Index 1 (100% over 32bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA253410.15625015414758114RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG252250.15553490937108774No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC248600.15328435468643176No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA247820.1528034142332724RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC240970.14857977051001392RNA PCR Primer, Index 1 (100% over 27bp)
GTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC240590.14834546618667988RNA PCR Primer, Index 2 (100% over 34bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA240210.14811116186334583No Hit
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC236290.14569412779105775RNA PCR Primer, Index 1 (100% over 26bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA232120.14312294613762888RNA PCR Primer, Index 1 (100% over 22bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC224850.13864033447805382RNA PCR Primer, Index 1 (100% over 33bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT222050.13691388156927664RNA PCR Primer, Index 9 (97% over 36bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT218170.1345215111099711RNA PCR Primer, Index 9 (97% over 36bp)
GTCTACGGCCATACCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC216520.1335041370744417RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTC210640.1298785859660096RNA PCR Primer, Index 34 (100% over 44bp)
TGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC210170.12958878851346484RNA PCR Primer, Index 1 (100% over 33bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC209390.1291078480603055RNA PCR Primer, Index 1 (100% over 31bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC208730.12870089844609373RNA PCR Primer, Index 1 (100% over 24bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA205870.12693745011784274No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC203710.12560561501678605RNA PCR Primer, Index 1 (100% over 33bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC202020.1245635773682741RNA PCR Primer, Index 1 (100% over 31bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT197390.12170876416554612RNA PCR Primer, Index 1 (100% over 30bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA197220.12160394381037036RNA PCR Primer, Index 1 (100% over 28bp)
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT193260.11916224612509978RNA PCR Primer, Index 9 (97% over 36bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC191010.1177749178948324RNA PCR Primer, Index 1 (100% over 27bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATG190300.11733713876439245RNA PCR Primer, Index 12 (97% over 37bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT186130.11476595711096356RNA PCR Primer, Index 1 (100% over 30bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC185670.11448232556166445RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG181110.1116706736816559RNA PCR Primer, Index 1 (100% over 29bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC180520.11130688539016356RNA PCR Primer, Index 1 (100% over 31bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATGGCATCTCG177350.10935229406129797RNA PCR Primer, Index 34 (100% over 45bp)
GTCTACGGCCATACCACCCTGAATGGAATTCTCGGGTGCCAAGGAACTCC176390.1087603673497172RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA173800.10716339840909832No Hit
TCCAGGGTTCATGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG172000.1060535358248844No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA170800.10531362743540847RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG167250.10312473178320883RNA PCR Primer, Index 1 (100% over 29bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA166010.10236015978075035RNA PCR Primer, Index 7 (100% over 35bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT163160.10060287735574501RNA PCR Primer, Index 1 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)