FastQCFastQC Report
Fri 10 May 2024
EGAF00002258483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30856462
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG533470.17288761102941744No Hit
CCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGGTCCCG495720.16065354479071514No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC423940.13739099446981315No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG372110.12059386458499358No Hit
GTCCCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCG339750.11010659614832055No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG325080.10535232457953216TruSeq Adapter, Index 18 (97% over 39bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC315730.10232216512703239No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA315090.10211475314311796No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC314830.10203049202465272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)