FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11708539
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGC6770295.78235252066889RNA PCR Primer, Index 31 (100% over 50bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA6231515.3221926322319115RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3424032.924387064859245RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA3258842.7833019986524365No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC1771411.5129214669738043No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC1410791.2049240302312696No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA1321471.1286378257782632No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1274681.0886755384254174RNA PCR Primer, Index 2 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1261181.0771454918500079RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG1025330.8757113077899814No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG883070.754210239210887Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC806050.6884291883043648No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG801830.6848249811526442No Hit
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG656590.5607787615517188No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC632030.5398026175597143No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA599040.5116266000395097RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT530520.45310520808787497RNA PCR Primer, Index 1 (100% over 30bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG518230.4426085953166317No Hit
CGCGACCTCAGATCAGACGTGGCTGGAATTCTCGGGTGCCAAGGAACTCC504300.4307112954058572RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC478160.40838570892576775RNA PCR Primer, Index 30 (100% over 36bp)
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA455210.3887846297475714RNA PCR Primer, Index 7 (100% over 35bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA433990.3706611046860757RNA PCR Primer, Index 1 (100% over 32bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA414310.35385285901170077RNA PCR Primer, Index 1 (100% over 28bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCGTATGCC405600.34641384377675133RNA PCR Primer, Index 31 (100% over 50bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA399910.34155414266459716RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA384770.3286234089496563RNA PCR Primer, Index 1 (100% over 28bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG383580.32760705669597207No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGA376560.32161143247675905RNA PCR Primer, Index 31 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG366310.31285713785468877No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC348590.29772288412755854No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG335970.2869444257733608No Hit
GTCTACGGCCATACCACCCTGAACTGGAATTCTCGGGTGCCAAGGAACTC334530.28571455413865043RNA PCR Primer, Index 1 (100% over 26bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC330150.2819736945830731RNA PCR Primer, Index 2 (100% over 34bp)
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC328760.28078652682456795RNA PCR Primer, Index 30 (100% over 36bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC321640.27470549485294454RNA PCR Primer, Index 30 (100% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC318310.2718614166976768RNA PCR Primer, Index 30 (100% over 36bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG311860.26635261666720333No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC309410.26426012673314747RNA PCR Primer, Index 1 (100% over 26bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC304080.25970789352967094RNA PCR Primer, Index 1 (100% over 27bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC300680.25680402994771595RNA PCR Primer, Index 1 (100% over 27bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA294410.2514489638715812RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA294280.2513379337934477RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC289770.24748604415973674RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT288930.24676861903948905No Hit
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA281220.240183681328644RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT281000.23999578427334103RNA PCR Primer, Index 1 (100% over 30bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA270700.23119878577506553RNA PCR Primer, Index 7 (100% over 35bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA269710.23035324902620216RNA PCR Primer, Index 7 (100% over 35bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC269070.22980663941077534RNA PCR Primer, Index 1 (100% over 26bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC260890.2228202852636012RNA PCR Primer, Index 30 (100% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT245170.20939418658467976No Hit
GTAGTCGTGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG242370.2070027695171874RNA PCR Primer, Index 31 (100% over 37bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC239210.20430388454101747RNA PCR Primer, Index 1 (100% over 27bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA237860.2031508798834765RNA PCR Primer, Index 1 (100% over 28bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC233380.19932461257548872RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTTGGAATTCTCGGGTGCCAA229540.19604495488292775No Hit
TTCACAGTGGCTAAGTTCTGCTGGAATTCTCGGGTGCCAAGGAACTCCAG225280.19240658463024293RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA221950.18956250647497525RNA PCR Primer, Index 1 (100% over 28bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT221370.18906714151099466RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA220140.18801662615634623RNA PCR Primer, Index 1 (100% over 32bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT219640.18758958739429402RNA PCR Primer, Index 1 (100% over 30bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC214670.18334482209949507RNA PCR Primer, Index 2 (100% over 34bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC211590.18071426332525348RNA PCR Primer, Index 1 (100% over 33bp)
CATTGCACTTGTCTCGGTCTGATGGAATTCTCGGGTGCCAAGGAACTCCA210140.17947585091530208RNA PCR Primer, Index 1 (100% over 28bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACG209440.178877996648429RNA PCR Primer, Index 31 (100% over 37bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA209360.17880967044650062RNA PCR Primer, Index 7 (100% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC208410.17799829679860144RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT208260.17787018516998576No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG201360.1719770502536653RNA PCR Primer, Index 1 (100% over 29bp)
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC197960.16907318667171028RNA PCR Primer, Index 2 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC195690.16713443069199327RNA PCR Primer, Index 1 (100% over 27bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA195060.16659636185180748RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA193960.1656568765752926RNA PCR Primer, Index 7 (100% over 35bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA192440.1643586787386539RNA PCR Primer, Index 7 (100% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG191420.1634875196640674No Hit
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC190390.16260781981423986RNA PCR Primer, Index 1 (100% over 31bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA189270.1616512529872429RNA PCR Primer, Index 1 (100% over 32bp)
TCCAGGGTTCATGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG187740.16034451437536315No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATA187250.159926016388552RNA PCR Primer, Index 31 (100% over 40bp)
GTCAGGATGGCCGAGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC185400.1583459729689588RNA PCR Primer, Index 2 (100% over 34bp)
TACCTGGTTGATCCTGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA181430.15495528519826426RNA PCR Primer, Index 1 (100% over 32bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA179780.153546057283492RNA PCR Primer, Index 1 (100% over 23bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA176330.15059948982533175RNA PCR Primer, Index 1 (100% over 28bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC175120.1495660560211654RNA PCR Primer, Index 1 (100% over 31bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA173780.1484215921388655RNA PCR Primer, Index 1 (100% over 32bp)
TGAGGTAGTAGGTTGTGTGGTTTGGAATTCTCGGGTGCCAAGGAACTCCA168690.14407433754117402RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA167120.14273343582833006No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG166440.14215266311193908No Hit
GTCTACGGCCATACCACCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTC163890.1399747654254728RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG160260.13687446401297376No Hit
CGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC157180.1342439052387322RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC152260.13004184382013845No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC150000.12811162861566247RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTC148380.12672802302661332RNA PCR Primer, Index 31 (100% over 44bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT146350.12499424565268136RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGATATCTCG143660.12269677711284047RNA PCR Primer, Index 31 (100% over 45bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC142450.12166334330867411RNA PCR Primer, Index 1 (100% over 31bp)
TCGTGGGTTCGTGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG141950.1212363045466219No Hit
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA141530.12087759198649807RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGTGGAATTCTCGGGTGCCAAGGAACTCCAG141460.12081780655981075RNA PCR Primer, Index 1 (100% over 29bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG141420.12078364345884658RNA PCR Primer, Index 1 (100% over 29bp)
CCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC141150.12055304252733838RNA PCR Primer, Index 2 (100% over 34bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCACGAT140190.11973312810419814RNA PCR Primer, Index 31 (100% over 39bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC139250.11893029523153999RNA PCR Primer, Index 1 (100% over 24bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC138080.11793102452833783RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGTGGAATTCTCGGGTGCCA137350.1173075479357416No Hit
GTCTACGGCCATACCACCCTGAATGGAATTCTCGGGTGCCAAGGAACTCC135960.11612038017723646RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134960.11526630265313205RNA PCR Primer, Index 1 (100% over 31bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG134750.11508694637307011RNA PCR Primer, Index 1 (100% over 29bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA134430.1148136415653567RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG133590.11409621644510898RNA PCR Primer, Index 1 (100% over 29bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC129470.11057741704579879RNA PCR Primer, Index 1 (100% over 31bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC126240.10781874664294153RNA PCR Primer, Index 1 (100% over 27bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT119850.10236119126391431RNA PCR Primer, Index 1 (100% over 30bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC118790.10145586908836364RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAC118380.1011056973034808RNA PCR Primer, Index 30 (100% over 36bp)

[FAIL]Adapter Content

Adapter graph