FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11849205
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA6846725.778210436902729RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG2844222.400346689925611RNA PCR Primer, Index 6 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2695242.27461673589072No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2339961.97478227442263No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2026891.7105704559926171No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGC1612231.3606229278673125RNA PCR Primer, Index 41 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA871770.7357202445227338No Hit
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC861460.7270192388434498RNA PCR Primer, Index 1 (100% over 27bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA815830.6885103262201979RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC753150.6356122625948323No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA744790.6285569369421831RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC741460.625746621819776RNA PCR Primer, Index 6 (97% over 36bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA739220.6238561996353342RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT730670.6166405256723975RNA PCR Primer, Index 1 (100% over 30bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC717850.6058212344203683RNA PCR Primer, Index 1 (100% over 33bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC691990.5839969854517666No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA637920.5383652320978496No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA551500.4654320690713006No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA521280.4399282483508387No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC485190.4094705087809689RNA PCR Primer, Index 6 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC380530.3211439079668214No Hit
GTAGTCGTGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGA360490.30423138092386787RNA PCR Primer, Index 31 (97% over 38bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA356110.3005349304025038RNA PCR Primer, Index 1 (100% over 28bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA332570.2806686186963598RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC331400.279681210680379No Hit
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG313590.2646506664371154RNA PCR Primer, Index 6 (100% over 34bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA313560.2646253482828595RNA PCR Primer, Index 7 (97% over 35bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA311450.2628446381001932RNA PCR Primer, Index 1 (100% over 28bp)
AGGCAAGATGCTGGCATAGCTGTGGAATTCTCGGGTGCCAAGGAACTCCA305460.2577894466337615RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG298270.25172152899709305No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA294540.24857363848460717RNA PCR Primer, Index 7 (97% over 35bp)
GTCTACGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA294130.2482276237097763RNA PCR Primer, Index 41 (100% over 40bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA293710.24787316955019342RNA PCR Primer, Index 41 (100% over 40bp)
TAGCTTATCAGACTGATGTTGACTTGGAATTCTCGGGTGCCAAGGAACTC289030.2439235374862702RNA PCR Primer, Index 1 (100% over 26bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG284630.24021020819540215Illumina Small RNA Adapter 2 (100% over 21bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG279150.23558542535132104RNA PCR Primer, Index 6 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA274950.23204088375549248No Hit
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG268960.22698569228906074No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG268720.2267831470550134Illumina Small RNA Adapter 2 (100% over 21bp)
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC262540.22156760727829422RNA PCR Primer, Index 6 (97% over 36bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA258370.21804838383672154RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG254770.21501020532601134No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG249820.21083270987378477No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG248380.20961743846950068RNA PCR Primer, Index 6 (100% over 34bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT248080.20936425692694152RNA PCR Primer, Index 1 (100% over 30bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG237550.20047758478311414No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC236640.19970960077068464RNA PCR Primer, Index 1 (100% over 26bp)
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG234420.19783605735574664RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA226140.19084824678111317RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT224940.1898355206108764No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA223260.188417703972545RNA PCR Primer, Index 1 (100% over 28bp)
TGAGAACTGAATTCCATAGGCTGTTGGAATTCTCGGGTGCCAAGGAACTC215770.18209660479331735RNA PCR Primer, Index 1 (100% over 26bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCGTATGCC212960.179725137678013RNA PCR Primer, Index 41 (100% over 50bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG211800.17874616904678414No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC208950.17634094439247192RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT208510.1759696114633851RNA PCR Primer, Index 1 (100% over 25bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC207120.17479653698286088RNA PCR Primer, Index 1 (100% over 31bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA206950.17465306744207734RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC199740.16856827103590494No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC196150.1655385319099467RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC190590.1608462339878498No Hit
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG187210.15799372194168299No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC184620.15580792129092205RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA181110.15284569724297958RNA PCR Primer, Index 7 (97% over 35bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC180880.15265159139368423No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC180170.15205239507629414No Hit
GTAGTCGTGGCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA175580.14817871747513864RNA PCR Primer, Index 7 (97% over 35bp)
TAGCTTATCAGACTGATGTTGTGGAATTCTCGGGTGCCAAGGAACTCCAG172270.14538528112223562RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG171940.14510678142542052Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA170090.14354549524630555No Hit
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC164010.13841434931710608RNA PCR Primer, Index 6 (97% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG161550.13633826066812077No Hit
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCT161000.13587409450676227RNA PCR Primer, Index 41 (100% over 43bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTCG160880.1357728218897386RNA PCR Primer, Index 41 (100% over 45bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA159680.13476009571950187RNA PCR Primer, Index 1 (100% over 28bp)
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA158770.13399211170707234RNA PCR Primer, Index 7 (97% over 35bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC151010.12744314913954144RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC142880.12058192933618753RNA PCR Primer, Index 1 (100% over 26bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACG141340.11928226408438372RNA PCR Primer, Index 31 (97% over 37bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG141160.1191303551588482RNA PCR Primer, Index 1 (100% over 29bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA140000.11815138652761938RNA PCR Primer, Index 1 (100% over 32bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA135690.1145140116995191No Hit
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA135590.11442961785199934RNA PCR Primer, Index 1 (100% over 28bp)
CGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG135330.11421019384844806RNA PCR Primer, Index 6 (100% over 34bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC134700.11367851260907376RNA PCR Primer, Index 1 (100% over 31bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA134590.11358567937680207RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC130690.11029431932353267RNA PCR Primer, Index 1 (100% over 27bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA130340.10999894085721362RNA PCR Primer, Index 1 (100% over 28bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC130140.10983015316217418RNA PCR Primer, Index 1 (100% over 31bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACATCTC129670.10943350207883146RNA PCR Primer, Index 41 (100% over 44bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA129620.10939130515507159RNA PCR Primer, Index 1 (100% over 28bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC129170.10901153284123281RNA PCR Primer, Index 1 (100% over 33bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC127890.10793129159298029RNA PCR Primer, Index 1 (100% over 24bp)
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA127430.10754307989438955RNA PCR Primer, Index 1 (100% over 28bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA126630.10686792911423172RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC126140.10645439926138506No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA125540.10594803617626668No Hit
CAACACCAGTCGATGGGCTGTTGGAATTCTCGGGTGCCAAGGAACTCCAG124710.10524756724185293RNA PCR Primer, Index 1 (100% over 29bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG123920.1045808558464471RNA PCR Primer, Index 1 (100% over 29bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGACGACA122970.10377911429500966RNA PCR Primer, Index 41 (100% over 40bp)
GTAGTCGTGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAC120390.10160175302900067RNA PCR Primer, Index 6 (97% over 36bp)
TCGTGGGTTCGTGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG120380.1015933136442487No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTTGGAATTCTCGGGTGCC119370.10074093578429945No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)