FastQCFastQC Report
Fri 10 May 2024
EGAF00002258495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32274380
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC11774253.6481723274002475TruSeq Adapter, Index 7 (100% over 50bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT627610.19446074564406812No Hit
CACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCG615120.19059080298366693No Hit
CCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGC460610.1427169166379029No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG419810.13007531050945054No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG399350.12373591684797663No Hit
CACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGG357050.11062954578833117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)