FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10594900
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA4388434.142021161124692RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1838581.7353443637976762RNA PCR Primer, Index 6 (100% over 34bp)
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1526601.440881933760583RNA PCR Primer, Index 1 (100% over 33bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC1086901.0258709379040858No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1059501.0000094385034308RNA PCR Primer, Index 6 (100% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA947580.8943737081048428No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC914170.8628396681422192RNA PCR Primer, Index 1 (100% over 27bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC906830.8559118066239417RNA PCR Primer, Index 6 (100% over 36bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA815150.7693796071694873RNA PCR Primer, Index 1 (100% over 28bp)
TTCACAGTGGCTAAGTTCTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT756430.713956715023266RNA PCR Primer, Index 1 (100% over 30bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC738760.6972788794608726RNA PCR Primer, Index 6 (100% over 50bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC699510.6602327534946059RNA PCR Primer, Index 5 (97% over 35bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC660420.6233376435832334RNA PCR Primer, Index 5 (97% over 35bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA641290.6052817865199295No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA491400.4638080585942293RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG473620.4470263994940962RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA462680.43670067674069596No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA434670.41026342863075627RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA417020.39360447007522487RNA PCR Primer, Index 1 (100% over 28bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT415560.3922264485743141RNA PCR Primer, Index 6 (100% over 39bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC406890.3840432660997272RNA PCR Primer, Index 1 (100% over 27bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC391900.3698949494568141RNA PCR Primer, Index 5 (97% over 35bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA380620.35924831758676345No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA380390.35903123200785286RNA PCR Primer, Index 1 (100% over 32bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC376460.3553219001595107RNA PCR Primer, Index 1 (100% over 26bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC376440.3553030231526489RNA PCR Primer, Index 6 (100% over 50bp)
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT355700.3357275670369706RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC344970.3256000528556192RNA PCR Primer, Index 1 (100% over 24bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG335660.316812806161455RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC333530.3148024049306742No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA332510.3138396775807228No Hit
TTTGGCAATGGTAGAACTCACACTTGGAATTCTCGGGTGCCAAGGAACTC329090.3106117094073564RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC326920.30856355416285197No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA326120.3078084738883803No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC323990.30579807265759945RNA PCR Primer, Index 6 (100% over 36bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC315700.29797355331338665RNA PCR Primer, Index 1 (100% over 33bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA309050.2916969485318408RNA PCR Primer, Index 1 (100% over 28bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG307270.2900168949211413RNA PCR Primer, Index 6 (100% over 34bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC303060.2860432849767341RNA PCR Primer, Index 1 (100% over 27bp)
CTCGTCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA298090.2813523487715788RNA PCR Primer, Index 6 (100% over 40bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA296090.2794646480853996RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC290250.27395256208175633No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA287110.270988872004455RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA286120.2700544601647963RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG273010.2576805821668916No Hit
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA272110.256831116858111RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC266540.25157387044710194RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC262740.24798723914336143No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC261840.2471377738345808RNA PCR Primer, Index 1 (100% over 26bp)
TCTGACACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA261200.24653370961500343RNA PCR Primer, Index 6 (100% over 40bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA260800.2461561694777676RNA PCR Primer, Index 1 (100% over 28bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA256320.2419277199407262No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC255560.2412103936799781RNA PCR Primer, Index 6 (100% over 44bp)
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG247010.23314047324656204RNA PCR Primer, Index 1 (100% over 29bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT240300.2268072374444308RNA PCR Primer, Index 1 (100% over 30bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC237830.22447592709699948No Hit
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA234940.22174819960547057RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG233600.2204834401457305Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC232010.21898271810021802RNA PCR Primer, Index 1 (100% over 31bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT227580.2148014610803311RNA PCR Primer, Index 1 (100% over 25bp)
TCACGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGCCATGGAATTCT226630.21390480325439598No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA221010.20860036432623244RNA PCR Primer, Index 1 (100% over 23bp)
TGTAAACATCCCCGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC216500.20434359927889836RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC215010.20293726226769485RNA PCR Primer, Index 6 (100% over 44bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC214690.20263523015790616No Hit
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA212380.2004549358653692RNA PCR Primer, Index 1 (100% over 32bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG211800.19990750266637722RNA PCR Primer, Index 6 (100% over 45bp)
CTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC211020.19917129939876735RNA PCR Primer, Index 6 (100% over 36bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA205620.1940745075460835RNA PCR Primer, Index 6 (100% over 38bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC201230.18993100453992015RNA PCR Primer, Index 6 (100% over 36bp)
AGATTTCAACTTAACTTGACCGCTCTGACCATGGAATTCTCGGGTGCCAA200470.18921367827917204No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC198740.18758081718562705No Hit
TCCTTCATTCCACCGGAGTCTTGGAATTCTCGGGTGCCAAGGAACTCCAG190450.17975629784141425RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC189620.1789729020566499RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG188400.17782140463808058No Hit
CCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC184520.17415926530689294RNA PCR Primer, Index 5 (97% over 35bp)
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG178170.16816581562827398No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA175710.16584394378427356No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC171950.16229506649425668RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGACTGGAATTCTCGGGT171510.16187977234329726No Hit
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA171380.16175707179869558RNA PCR Primer, Index 1 (100% over 32bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG171210.16159661724037036No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG169330.1598221785953619No Hit
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC168970.15948239247184967RNA PCR Primer, Index 1 (100% over 31bp)
TTTGGCAATGGTAGAACTCACATGGAATTCTCGGGTGCCAAGGAACTCCA165620.1563204938224995RNA PCR Primer, Index 1 (100% over 28bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCCATGGAATTCTCGGGTGCCAAG164640.1553955204862717No Hit
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC160180.15118594795609208No Hit
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA158800.14988343448262845RNA PCR Primer, Index 1 (100% over 32bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG158410.14951533284882348RNA PCR Primer, Index 1 (100% over 29bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT156430.14764650916950608No Hit
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA151800.14327648208100124RNA PCR Primer, Index 1 (100% over 28bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT149200.1408224711889683RNA PCR Primer, Index 6 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG149040.14067145513407395No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA148820.14046380805859424RNA PCR Primer, Index 6 (100% over 37bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCG148670.14032223050713077RNA PCR Primer, Index 6 (100% over 45bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA148400.1400673909144966RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC146510.13828351376605727RNA PCR Primer, Index 1 (100% over 27bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGCC146500.13827407526262636No Hit
CTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA142530.13452698940056063RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC142010.13403618722215407RNA PCR Primer, Index 5 (97% over 35bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA138140.1303834863943973RNA PCR Primer, Index 1 (100% over 23bp)
TCCAGGGTTCAGGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG136760.12908097292093365No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA134010.12648538447743726No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT133220.12573974270639646RNA PCR Primer, Index 1 (100% over 25bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA132500.12506017045937196RNA PCR Primer, Index 6 (100% over 37bp)
TACCCTGTAGATCCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA130940.12358776392415219RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGCATTGTACAGGGCTATGATGGAATTCTCGGGTGCCAAGGAACTCC127710.12053912731597279RNA PCR Primer, Index 1 (100% over 27bp)
TGAGATGAAGCACTGTAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAG126160.1190761592841839RNA PCR Primer, Index 1 (100% over 29bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG125750.11868918064351718No Hit
TCAGTGCACTACAGAACTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA124280.11730172063917546RNA PCR Primer, Index 1 (100% over 28bp)
TGTTCGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA122590.11570661355935404RNA PCR Primer, Index 6 (100% over 37bp)
CGGGCCACCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATA122330.11546121247015073RNA PCR Primer, Index 6 (100% over 40bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC120950.11415869899668708RNA PCR Primer, Index 6 (100% over 36bp)
TACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT120750.11396992892806918RNA PCR Primer, Index 1 (100% over 30bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA119740.11301664008154866No Hit
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTC118760.11209166674532087RNA PCR Primer, Index 6 (100% over 44bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA118400.1117518806218086RNA PCR Primer, Index 1 (100% over 22bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA118230.11159142606348338No Hit
TGTAAACATCCTTGACTGGAAGCTTGGAATTCTCGGGTGCCAAGGAACTC117530.11093073082332064RNA PCR Primer, Index 1 (100% over 26bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC117460.11086466129930438RNA PCR Primer, Index 1 (100% over 31bp)
ATCTAAAGGTCCCTGGTTCGATCCCGGGTTTCGGCACCATGGAATTCTCG115620.10912797666801952No Hit
TCGACACAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC115440.1089580836062634RNA PCR Primer, Index 1 (100% over 33bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA114990.10853335095187308RNA PCR Primer, Index 1 (100% over 22bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG114580.10814637231120634RNA PCR Primer, Index 1 (100% over 29bp)
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCATGGAATTCT113030.10668340427941744No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCATGGAATTCTCGGGTGCCAAG112960.10661733475540117No Hit
TGAGATGAAGCACTGTAGCTCTTGGAATTCTCGGGTGCCAAGGAACTCCA110620.10440872495257152RNA PCR Primer, Index 1 (100% over 28bp)
GAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGC108640.10253990127325412No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG107880.10182257501250601No Hit
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA107810.10175650548848975RNA PCR Primer, Index 1 (100% over 23bp)
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTTGGAATTCTCGGGTGCC107530.10149222739242465No Hit
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG107280.10125626480665226No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG106190.1002274679326846RNA PCR Primer, Index 6 (100% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)