FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9090167
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT6506677.1579213011158105RNA PCR Primer, Index 9 (100% over 36bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA5170835.688377342242448RNA PCR Primer, Index 1 (100% over 32bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGC2289502.518655597856453RNA PCR Primer, Index 9 (100% over 50bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1949642.144779078316163RNA PCR Primer, Index 6 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA1881702.0700389772817154RNA PCR Primer, Index 7 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG1784741.9633742702416797RNA PCR Primer, Index 6 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1589171.7482297079910634No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA1042681.1470416330085025No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC939481.033512365614405No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC784510.8630314492572029RNA PCR Primer, Index 1 (100% over 33bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCGTATGCC618040.679899500196201RNA PCR Primer, Index 9 (100% over 50bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC563080.6194385647700422RNA PCR Primer, Index 1 (100% over 33bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA503320.5536971983022975RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA477870.5256999128838887No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA443950.48838486685668153No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA367530.40431600431543224RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA337400.37117029863147727No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA326270.3589262991537999No Hit
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG325150.35769419857742984Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGAT316610.34829943168260824RNA PCR Primer, Index 9 (100% over 36bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA296350.3260116123279143RNA PCR Primer, Index 1 (100% over 32bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG282480.3107533667973317RNA PCR Primer, Index 6 (100% over 34bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTC278250.3060999869419341RNA PCR Primer, Index 9 (100% over 44bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC273140.30047852806224573No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC251780.27698060992718837No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC247680.27247024174583373No Hit
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA244100.26853192026065087RNA PCR Primer, Index 1 (100% over 32bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA236910.2606222745962753RNA PCR Primer, Index 7 (97% over 35bp)
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTTGGAATTCTCGGGTGC235300.25885113001774335No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGA233320.25667295221308917RNA PCR Primer, Index 7 (97% over 35bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC229540.25251461276784026RNA PCR Primer, Index 1 (100% over 27bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGATCTCG214200.23563923523077188RNA PCR Primer, Index 9 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC211820.23302102150598555No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC190480.2095451051669348RNA PCR Primer, Index 1 (100% over 26bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATCAGA188400.20725691838224752RNA PCR Primer, Index 9 (100% over 40bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA184330.2027795528949028RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA180760.19885223230772328No Hit
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG179170.19710308952519792Illumina Small RNA Adapter 2 (100% over 21bp)
CTGGACGCGAGCCGGGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT177640.19541995213069244RNA PCR Primer, Index 1 (100% over 30bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC177260.19500191800656688RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG173850.1912506117874402Illumina Small RNA Adapter 2 (100% over 21bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG163290.17963366349595117Illumina Small RNA Adapter 2 (100% over 21bp)
GTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG162580.17885259973771658RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG158280.17412221359629587No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC142780.15707082169117464RNA PCR Primer, Index 1 (100% over 24bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA142490.15675179564907882RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG141970.15617974895290704No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT141340.1554866923786989No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT141150.15527767531663608RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG140200.1542325900063222No Hit
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC139640.15361653971813718RNA PCR Primer, Index 1 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT139580.15355053433011737No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG137910.1517133843635656No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA137890.15169138256755899RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA129100.14202159322265479RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA128420.14127353215843008No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGATC128410.1412625312604268RNA PCR Primer, Index 9 (100% over 37bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC127690.1404704666041889No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC125860.13845730226958428No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA125160.13768723940935298RNA PCR Primer, Index 1 (100% over 28bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT124810.13730220797923734RNA PCR Primer, Index 1 (100% over 30bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA123550.13591609483082104RNA PCR Primer, Index 1 (100% over 32bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC123130.13545405711468228No Hit
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC121780.13396893588423622No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC120850.13294585236992895No Hit
AGTTGGTCCGAGTGTTGTGGGTTATTGTTAAGTTGGAATTCTCGGGTGCC116500.12816046173849172No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG109080.11999779542004013RNA PCR Primer, Index 6 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC107030.11774261132936283No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA106370.11701655206114475RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC105790.11637849997695313No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC105620.11619148471089695RNA PCR Primer, Index 1 (100% over 27bp)
CGAGAACTTTGAAGGCCGAAGTGGAATTCTCGGGTGCCAAGGAACTCCAG104170.11459635450041789RNA PCR Primer, Index 1 (100% over 29bp)
TCGTGGGTTCGAGCCCCACGTTGGGCGCTGGAATTCTCGGGTGCCAAGGA98480.10833684353653789RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTCTGGAATTCTCGGGTGC96440.10609266034386387No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTTTGGAATTCTCGGGTGCC95520.10508057772755991No Hit
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC94830.10432151576533194RNA PCR Primer, Index 1 (100% over 33bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG92160.10138427599844975No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)