FastQCFastQC Report
Mon 12 Sep 2022
EGAF00002258553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15194714
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC6087064.00603788922911RNA PCR Primer, Index 1 (100% over 33bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC4597973.0260326058127847RNA PCR Primer, Index 13 (100% over 50bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGCC4393552.8914989778682245RNA PCR Primer, Index 13 (100% over 50bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT3621012.3830721657544855RNA PCR Primer, Index 8 (97% over 36bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT3351872.2059447779010517RNA PCR Primer, Index 8 (97% over 36bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA2717211.7882600488564642RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA2035651.3397093225973191No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC1894551.2468480815104517RNA PCR Primer, Index 13 (100% over 44bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1564461.0296080597502526RNA PCR Primer, Index 1 (100% over 34bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1545991.0174525167107458RNA PCR Primer, Index 2 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA1490190.9807292193851099No Hit
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1444400.950593739375417RNA PCR Primer, Index 2 (97% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1263660.8316444784679725RNA PCR Primer, Index 1 (100% over 34bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1093280.7195133781392661RNA PCR Primer, Index 1 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG1046970.6890356738534203RNA PCR Primer, Index 2 (97% over 35bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC771680.5078608258108708No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC681200.4483138017602701RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC639120.4206199603362064No Hit
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA629020.4139729118955447RNA PCR Primer, Index 1 (100% over 34bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA592200.389740800649489No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC578990.38104698778798995No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA573440.37739440176366595No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA563550.3708855592806814No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT550790.3624879020427762No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA518360.3411449534357804RNA PCR Primer, Index 1 (100% over 28bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA515380.3391837450839812RNA PCR Primer, Index 1 (100% over 32bp)
GACCCGGGTTCGTTTCCCGGCCAACGCACCATGGAATTCTCGGGTGCCAA511310.33650518199947693No Hit
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA505940.3329710582245905RNA PCR Primer, Index 1 (100% over 32bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC503580.3314178865097428RNA PCR Primer, Index 1 (100% over 24bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA503300.3312336118995066RNA PCR Primer, Index 13 (100% over 39bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC494860.32567904864810227RNA PCR Primer, Index 1 (100% over 31bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA494300.3253104994276299RNA PCR Primer, Index 13 (100% over 38bp)
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG493910.32505383122051523RNA PCR Primer, Index 13 (100% over 45bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAA481150.31665617398261003RNA PCR Primer, Index 13 (100% over 39bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA465770.30653423289178067No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC449870.2960700675247984RNA PCR Primer, Index 1 (100% over 26bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA437290.28779087253633073RNA PCR Primer, Index 13 (100% over 38bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA411280.27067307749260694RNA PCR Primer, Index 1 (100% over 28bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT408900.2691067433055996RNA PCR Primer, Index 8 (97% over 36bp)
CATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTAT408420.2687908439737661RNA PCR Primer, Index 13 (100% over 48bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC387860.2552598225935677No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG380510.2504226140748684No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC349620.2300931758241715RNA PCR Primer, Index 1 (100% over 24bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC343040.2257627224836216RNA PCR Primer, Index 1 (100% over 33bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA337250.22195218679338088RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC329840.21707549085820238RNA PCR Primer, Index 1 (100% over 33bp)
GTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC329370.21676617276244883RNA PCR Primer, Index 13 (100% over 44bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC318890.20986903735075238RNA PCR Primer, Index 1 (100% over 27bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC315290.20749979236200167RNA PCR Primer, Index 1 (100% over 26bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC306620.2017938606807604RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA305700.20118838696141303No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA299160.19688425856518257RNA PCR Primer, Index 1 (100% over 23bp)
CCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTA291200.19164559464561162RNA PCR Primer, Index 13 (100% over 47bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA286300.18842078896647874RNA PCR Primer, Index 1 (100% over 28bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA282110.18566325104901613RNA PCR Primer, Index 1 (100% over 28bp)
TCGTACGACTCTTAGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA282070.18563692610469668RNA PCR Primer, Index 1 (100% over 32bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC280930.1848866651915923RNA PCR Primer, Index 13 (100% over 37bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA274320.18053646814280283No Hit
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC269840.17758807437902419RNA PCR Primer, Index 1 (100% over 26bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA268610.17677858234120103RNA PCR Primer, Index 1 (100% over 32bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG259500.1707830762724458Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT258710.17026315862213662RNA PCR Primer, Index 8 (97% over 36bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG257660.169572128833751Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAG251390.1654456938116769RNA PCR Primer, Index 2 (97% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC241160.15871308930197697RNA PCR Primer, Index 13 (100% over 37bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTC241090.15866702064941796RNA PCR Primer, Index 13 (100% over 44bp)
CAAACGAGAACTTTGAAGGCCGAATGGAATTCTCGGGTGCCAAGGAACTC240070.1579957345692719RNA PCR Primer, Index 1 (100% over 26bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC233100.15340861302160738RNA PCR Primer, Index 13 (100% over 37bp)
TAGCTTATCAGACTGATGTTGATGGAATTCTCGGGTGCCAAGGAACTCCA231000.15202655344483615RNA PCR Primer, Index 1 (100% over 28bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG228540.15040756936918984RNA PCR Primer, Index 1 (100% over 29bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA222980.146748402108786No Hit
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT222980.146748402108786RNA PCR Primer, Index 1 (100% over 30bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCG219550.14449103813339298RNA PCR Primer, Index 13 (100% over 45bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA219410.1443989008282749RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTC218890.144056676552122RNA PCR Primer, Index 13 (100% over 37bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAA217580.14319453462565995RNA PCR Primer, Index 13 (100% over 40bp)
GTCTACGGCCATACCACCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT214610.14123990750994062RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG211960.1394958799487769Illumina Small RNA Adapter 2 (100% over 21bp)
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG211480.1391799806169435RNA PCR Primer, Index 1 (100% over 29bp)
GAAGGATCATTATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCA210970.13884433757687048RNA PCR Primer, Index 13 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG205760.13541551357926185No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAA204420.1345336279445602RNA PCR Primer, Index 1 (100% over 23bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT203530.13394789793345238RNA PCR Primer, Index 1 (100% over 25bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA201490.1326053257731603RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCT200450.13192087722085458RNA PCR Primer, Index 13 (100% over 43bp)
GCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGA200000.13162472159726074RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA199780.13147993440350375RNA PCR Primer, Index 1 (100% over 23bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG197410.1299201814525762No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT195370.12857760929228415RNA PCR Primer, Index 1 (100% over 30bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC185090.12181209860218495No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG183700.12089730678708398No Hit
CGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA182500.1201075584575004RNA PCR Primer, Index 1 (100% over 34bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA180880.1190413982125626RNA PCR Primer, Index 1 (100% over 23bp)
GGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACT178080.11719865211020095RNA PCR Primer, Index 1 (100% over 25bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC175690.11562573668711369RNA PCR Primer, Index 1 (100% over 27bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT170090.11194024448239039RNA PCR Primer, Index 1 (100% over 25bp)
CCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAG167410.1101764732129871No Hit
CACGTTGGGCGCCATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGT167020.10991980500587244RNA PCR Primer, Index 8 (97% over 36bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC165800.10911689420412915RNA PCR Primer, Index 1 (100% over 27bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA164670.10837321452710463RNA PCR Primer, Index 1 (100% over 32bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG162440.10690559888129517No Hit
GTCTACGGCCATACCACCCTGATGGAATTCTCGGGTGCCAAGGAACTCCA161150.10605661942699282RNA PCR Primer, Index 1 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTAT158400.10424677950503049RNA PCR Primer, Index 13 (100% over 48bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA157090.10338463757856844No Hit
CTCGTCGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAAT157030.10334515016208927RNA PCR Primer, Index 13 (100% over 41bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC156660.10310164442713433No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATG156020.1026804453180231RNA PCR Primer, Index 13 (100% over 49bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA155900.10260147048506475RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG155440.10229873362539105No Hit
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA154910.1019499281131583RNA PCR Primer, Index 1 (100% over 28bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA152820.10057444977246692RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)