FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10976076
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC4275903.895654512596305RNA PCR Primer, Index 1 (100% over 33bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG4163213.792985762853683RNA PCR Primer, Index 7 (97% over 36bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG2668962.431615816071244RNA PCR Primer, Index 7 (97% over 36bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC2117561.929250489883634RNA PCR Primer, Index 16 (100% over 50bp)
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA1819921.65807889814174RNA PCR Primer, Index 1 (100% over 32bp)
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC1443681.3152970150716887RNA PCR Primer, Index 5 (97% over 35bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1382471.2595302729317837RNA PCR Primer, Index 2 (100% over 34bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC1118401.0189433819518015RNA PCR Primer, Index 5 (97% over 35bp)
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA908510.8277183940781752RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA896820.817067957619827RNA PCR Primer, Index 1 (100% over 28bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC885410.8066726214359302RNA PCR Primer, Index 2 (100% over 34bp)
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC874050.7963228388724714RNA PCR Primer, Index 1 (100% over 27bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC743160.6770725712905049RNA PCR Primer, Index 5 (97% over 35bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA719090.6551430584117676RNA PCR Primer, Index 1 (100% over 23bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC690560.629150162589982RNA PCR Primer, Index 2 (100% over 34bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC600470.5470716492852272RNA PCR Primer, Index 1 (100% over 26bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC552940.5037683776970933RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC529540.4824492833322218RNA PCR Primer, Index 1 (100% over 31bp)
GGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC457050.4164056444215583RNA PCR Primer, Index 2 (100% over 34bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA439950.4008263062318446No Hit
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA431470.3931004122056006RNA PCR Primer, Index 1 (100% over 28bp)
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA431380.39301841568881263RNA PCR Primer, Index 1 (100% over 22bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA419680.38235886850637696No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGCC414990.37808593890931513RNA PCR Primer, Index 16 (100% over 50bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT412660.3759631401969155RNA PCR Primer, Index 1 (100% over 25bp)
GCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA407980.3716993213239413RNA PCR Primer, Index 1 (100% over 32bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC392540.35763236333276116RNA PCR Primer, Index 1 (100% over 26bp)
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC380260.3464443941532475RNA PCR Primer, Index 1 (100% over 24bp)
CTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC338770.30864399991399477RNA PCR Primer, Index 16 (100% over 38bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC325000.29609853284543586RNA PCR Primer, Index 1 (100% over 33bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA321210.29264556841625367No Hit
GTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC311840.28410881994621756RNA PCR Primer, Index 16 (100% over 44bp)
TCGCTGGTTCGATTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG289330.2636005800251383Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC289170.2634548084397375RNA PCR Primer, Index 16 (100% over 39bp)
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG274250.2498616081011101No Hit
AATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAG269430.24547023909091006RNA PCR Primer, Index 1 (100% over 29bp)
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA262150.23883763195517235RNA PCR Primer, Index 1 (100% over 23bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA254690.23204103178585864RNA PCR Primer, Index 1 (100% over 28bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA254220.23161282775374367RNA PCR Primer, Index 1 (100% over 32bp)
CCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA253320.230792862585864RNA PCR Primer, Index 1 (100% over 32bp)
CCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC239060.21780097003701507RNA PCR Primer, Index 1 (100% over 33bp)
CAAGGGTTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCC238370.21717233007497397RNA PCR Primer, Index 16 (100% over 39bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC234680.21381047288666732No Hit
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA232850.21214321037864534RNA PCR Primer, Index 1 (100% over 28bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA232330.21166945272609264RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA222020.20227629619182663No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT220540.20092790902686897RNA PCR Primer, Index 1 (100% over 30bp)
TCGCTGGTTCGTTTCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG211960.19311090775974948Illumina Small RNA Adapter 2 (100% over 21bp)
TGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTC209930.19126143076997645RNA PCR Primer, Index 16 (100% over 38bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC205650.18736204086050426No Hit
ATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAG197740.1801554581072507RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC195020.1776773411554366RNA PCR Primer, Index 1 (100% over 27bp)
CTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCA191310.17429726251895486RNA PCR Primer, Index 1 (100% over 28bp)
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC190950.173969276451803RNA PCR Primer, Index 1 (100% over 24bp)
TAGCAGCACGTAAATATTGGCGTGGAATTCTCGGGTGCCAAGGAACTCCA186170.16961435033795322RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG180190.16416613733359717RNA PCR Primer, Index 1 (100% over 29bp)
CGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAA177130.16137825576280632RNA PCR Primer, Index 1 (100% over 23bp)
TCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT176220.16054917987083908RNA PCR Primer, Index 16 (100% over 37bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT176040.16038518683726316No Hit
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC175670.16004809004602372RNA PCR Primer, Index 1 (100% over 27bp)
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG175340.15974743615113451No Hit
TTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG174000.15852659912340256RNA PCR Primer, Index 16 (100% over 45bp)
CCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA173130.15773396612778554RNA PCR Primer, Index 1 (100% over 32bp)
TACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAG171920.15663156851319177RNA PCR Primer, Index 1 (100% over 29bp)
GGGTGCTGTAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG169810.15470920573071834RNA PCR Primer, Index 7 (97% over 36bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC164320.14970741820665237No Hit
AATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCA164010.14942498575993826RNA PCR Primer, Index 1 (100% over 28bp)
GTTCGATTCCCCGACGGGGAGCCATGGAATTCTCGGGTGCCAAGGAACTC163580.1490332246241735RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG160140.14589913553805567RNA PCR Primer, Index 1 (100% over 29bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA159610.14541626716141542RNA PCR Primer, Index 1 (100% over 28bp)
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA158690.14457808054536067RNA PCR Primer, Index 1 (100% over 28bp)
TCGCTGGTTCGAATCCGGCTCGAAGGACCTGGAATTCTCGGGTGCCAAGG158500.14440497678769718Illumina Small RNA Adapter 2 (100% over 21bp)
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA157350.1433572435176287RNA PCR Primer, Index 1 (100% over 28bp)
TGAGGTAGTAGTTTGTGCTGTTTGGAATTCTCGGGTGCCAAGGAACTCCA155830.14197241345632083RNA PCR Primer, Index 1 (100% over 28bp)
GAATACCGGGTGCTGTAGGCTTATGGAATTCTCGGGTGCCAAGGAACTCC153160.13953985012494446RNA PCR Primer, Index 1 (100% over 27bp)
ACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA152890.13929386057458057RNA PCR Primer, Index 1 (100% over 32bp)
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA152470.1389112101629034RNA PCR Primer, Index 1 (100% over 28bp)
CCCATAAAGTAGAAAGCACTTGGAATTCTCGGGTGCCAAGGAACTCCAGT152210.13867433133662704RNA PCR Primer, Index 1 (100% over 30bp)
CCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC151290.1378361447205723RNA PCR Primer, Index 2 (100% over 34bp)
GGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCG151190.13774503747969674RNA PCR Primer, Index 7 (97% over 36bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT149080.1358226746972233RNA PCR Primer, Index 1 (100% over 25bp)
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA145350.13242437461256645No Hit
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCA138160.12587376399361666RNA PCR Primer, Index 16 (100% over 40bp)
GTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC137500.12527245620383823RNA PCR Primer, Index 5 (97% over 35bp)
CCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGA136550.12440693741552081RNA PCR Primer, Index 1 (100% over 22bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG135050.12304032880238802Illumina Small RNA Adapter 2 (100% over 21bp)
GTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT130000.11843941313817434RNA PCR Primer, Index 16 (100% over 37bp)
TGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAAC128520.11709102597321666RNA PCR Primer, Index 1 (100% over 24bp)
CGGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTC128050.11666282194110172RNA PCR Primer, Index 16 (100% over 44bp)
GGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCC124800.11370183661264736RNA PCR Primer, Index 5 (97% over 35bp)
TGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT124500.1134285148900208RNA PCR Primer, Index 16 (100% over 37bp)
ACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC122850.11192524541557476RNA PCR Primer, Index 1 (100% over 31bp)
TAGCTTATCAGACTGATGTTGACTGGAATTCTCGGGTGCCAAGGAACTCC122760.11184324889878677RNA PCR Primer, Index 1 (100% over 27bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGT121400.11060419042287972RNA PCR Primer, Index 16 (100% over 37bp)
TTACTTGATGACAATAAAATATCTGATATGGAATTCTCGGGTGCCAAGGA120650.10992088611631334RNA PCR Primer, Index 1 (100% over 22bp)
GTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGGAA120360.10965667511777433RNA PCR Primer, Index 1 (100% over 23bp)
TACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC120090.10941068556741043RNA PCR Primer, Index 1 (100% over 31bp)
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC119440.10881848850171957RNA PCR Primer, Index 1 (100% over 27bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC117520.10706922947690958No Hit
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA116780.10639503589443075RNA PCR Primer, Index 1 (100% over 32bp)
GTTCGATTCCCCGACGGGGAGCCTGGAATTCTCGGGTGCCAAGGAACTCC116320.10597594258640336RNA PCR Primer, Index 1 (100% over 27bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA115130.1048917664199847RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCG113970.10383492242582869RNA PCR Primer, Index 16 (100% over 45bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC113240.1031698395674374No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT112890.10285096422437308No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC112400.10240453874408303No Hit

[FAIL]Adapter Content

Adapter graph