FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002258564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002258564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8980688
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA7152937.964790670826111RNA PCR Primer, Index 1 (100% over 32bp)
GTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC3632034.044266987117245RNA PCR Primer, Index 2 (100% over 34bp)
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC2304082.5655940836604056No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC1828652.036202571562446RNA PCR Primer, Index 1 (97% over 36bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA1043231.1616370594324177No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA1007781.1221634689903492No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA863720.9617525962376157No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC683270.7608214426333484RNA PCR Primer, Index 2 (100% over 34bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGC649040.7227063227227134RNA PCR Primer, Index 40 (100% over 50bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA638740.7112372682360193No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC607290.6762176795363563No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG551330.6139061951601035No Hit
TACCCTGTAGAACCGAATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCA550200.612647939667874RNA PCR Primer, Index 1 (100% over 28bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT550140.6125811296417379RNA PCR Primer, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCTGGAATTCT546900.6089733882303895No Hit
CCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC500490.5572958330141299RNA PCR Primer, Index 1 (100% over 33bp)
TTGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA495190.5513942807054426RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC494080.550158295221925No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTGGAATTCTCGGGTGC447850.49868117008407375No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT446260.4969107043914675No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATTGGAATTCTCGGGTGCC444550.49500661864658924No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGGAATTCTCGGGTGCCA444310.49473937854204486No Hit
TTTCTATGATGAATCAAACTAGCTCACTATGACCGACAGTGAAAATACAT432250.4813105632886923No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAT382550.4259695916393042No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGTGGAATTCT376480.4192106439952039No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA371540.4137099518433332No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC358700.39941260625021163No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA352730.3927650086496714No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG351780.3917071832358501No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTTGGA347070.3864625961841676No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCA341700.38048309884498827No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGGAATTCTCG305390.34005189802830255No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA294770.328226523402216No Hit
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC290330.32328258146814587RNA PCR Primer, Index 1 (100% over 26bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGTGGAATTCTC288420.32115579563614727No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG283350.3155103484276483No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC283120.31525424332746RNA PCR Primer, Index 1 (100% over 27bp)
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAATCTCGTATGCC281660.313628532691482RNA PCR Primer, Index 40 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCC280070.3118580669988758No Hit
CCTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGC276050.30738179524775827No Hit
TCCTTCATTCCACCGGAGTCTGTGGAATTCTCGGGTGCCAAGGAACTCCA264230.2942202200989501RNA PCR Primer, Index 1 (100% over 28bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAATGGAATTCTCG258050.28733878740693364No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAA257270.28647025706716456No Hit
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG245630.27350911199676464Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATGGAATTC244370.27210610144790687No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC232550.2589445262990987RNA PCR Primer, Index 1 (100% over 26bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTTGGAATTCTC228030.2539115043301805No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT227360.25316545903832754RNA PCR Primer, Index 1 (100% over 25bp)
TCGTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA217890.24262060991318257RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATGGAATTC213560.23779915302702867No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTC213340.23755418293119637RNA PCR Primer, Index 1 (97% over 36bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTGGAATTCTCGGGTGCCAAG208840.23254343097099017No Hit
TTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTT207340.23087318031758813No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTTGGAATTCTCGG206350.2297708148863428No Hit
GCATTGTGGTTCAGTGGTAGAATTCTCGCCTTGGAATTCTCGGGTGCCAA201100.2239249375994356No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC195820.21804565529946035RNA PCR Primer, Index 1 (100% over 33bp)
GCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGAA191080.21276766323470986RNA PCR Primer, Index 1 (100% over 23bp)
TATTGCACTTGTCCCGGCCTGTTGGAATTCTCGGGTGCCAAGGAACTCCA180720.20123179872187968RNA PCR Primer, Index 1 (100% over 28bp)
CTGGATGATGATAAGCAAATGCTGACTGAACATGAAGGTCTTAATTAGCT179110.19943906302056147No Hit
TGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT178780.199071607876813RNA PCR Primer, Index 1 (97% over 35bp)
ACAGATGATGAACTTATTGACGGGCGGACAGAAACTGTGTGCTGATTGTC178260.19849258765030028No Hit
GAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCA170820.19020814440942607RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTTGGAATTCTCGGGTG168080.18715715321587834No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCAA163840.18243591136892853No Hit
GAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTCC158760.17677932915607358RNA PCR Primer, Index 1 (100% over 27bp)
GCTTCTGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCC155260.17288207763146876No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTGGAA153910.17137885204340692No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAA153420.17083323682996224RNA PCR Primer, Index 1 (100% over 23bp)
CTCGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC150160.1672032254099018RNA PCR Primer, Index 1 (100% over 31bp)
TTCAAGTAATCCAGGATAGGCTTGGAATTCTCGGGTGCCAAGGAACTCCA148990.16590042990024817RNA PCR Primer, Index 1 (100% over 28bp)
GGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT147560.1643081242773382RNA PCR Primer, Index 1 (97% over 35bp)
AACATTCAACGCTGTCGGTGAGTGGAATTCTCGGGTGCCAAGGAACTCCA146160.1627492236674963RNA PCR Primer, Index 1 (100% over 28bp)
TCCTTCATTCCACCGGAGTCTGTTGGAATTCTCGGGTGCCAAGGAACTCC145690.16222587846276365RNA PCR Primer, Index 1 (100% over 27bp)
GTTTCCGTAGTGTAGTGGTCATCACGTTCGCCTTGGAATTCTCGGGTGCC145320.16181388330159116No Hit
GGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAAC139370.155188555709763RNA PCR Primer, Index 1 (100% over 24bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCTGGAATTCTCGGGT139160.15495472061828672No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA137950.15360738509120905No Hit
ACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTT137700.1533290099823087No Hit
GCACTTGCGGCCCCGGGTTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGC137290.15287247480371213No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA135270.15062320392379736RNA PCR Primer, Index 1 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATGGAAT133800.14898635828346335No Hit
GTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTT131110.14599104211169567No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGCTGGAATTCTCGGGTGC130330.1451225117719266No Hit
AAGCTGCCAGTTGAAGAACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA129430.14412036137988538RNA PCR Primer, Index 1 (100% over 28bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGCTTGGAATTCTCGGGTGCCA125000.13918755445017128No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTGGAATTCTCGGGTGCC122440.1363369933350318No Hit
CTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAAC122370.1362590483045397RNA PCR Primer, Index 1 (100% over 24bp)
GTTTGTGATGACTTACATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC120780.13448858261193353RNA PCR Primer, Index 1 (100% over 33bp)
CGTACGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC119980.13359778226345242RNA PCR Primer, Index 1 (100% over 33bp)
CTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC118230.13164915650115003RNA PCR Primer, Index 2 (100% over 34bp)
GCATGGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG117610.13095878623107718No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTATGG117360.13068041112217682No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCTGGAATTCTCGGGTGCCAAGGA113650.12654932450609577RNA PCR Primer, Index 1 (100% over 22bp)
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCTGGAATTCTC111980.12468977877864146No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATTGGAATT111690.12436686365231706No Hit
TCCAGGGTTCAAGTCCCTGTTCGGGCGCCATGGAATTCTCGGGTGCCAAG110660.12321995820364766No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATTGGAATT110200.12270774800327103No Hit
GTATGACTCTTAGCGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC109100.12148289752410951RNA PCR Primer, Index 2 (100% over 34bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG109050.12142722250232944Illumina Small RNA Adapter 2 (100% over 21bp)
AACATTCAACGCTGTCGGTGAGTTGGAATTCTCGGGTGCCAAGGAACTCC107720.11994626692297962RNA PCR Primer, Index 1 (100% over 27bp)
CGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTC105420.11738521592109646RNA PCR Primer, Index 1 (100% over 31bp)
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCCATGGAATTCT104470.11632739050727516No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC102540.11417833466656452No Hit
CAACGGAATCCCAAAAGCAGCTGTGGAATTCTCGGGTGCCAAGGAACTCC102480.11411152464042844RNA PCR Primer, Index 1 (100% over 27bp)
GTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTCAGAA101870.11343228937471161RNA PCR Primer, Index 40 (100% over 40bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTGGAAT101430.112942349183047No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT97410.1084660774319295RNA PCR Primer, Index 1 (100% over 25bp)
TGAGATGAAGCACTGTAGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGT97050.10806521727511299RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATT96640.10760868209651643No Hit
ATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCCAGT95520.10636156160864291RNA PCR Primer, Index 1 (100% over 30bp)
TGAATCTGACAACAGAGGCTTACGACCCCTTATTTACCCTGGAATTCTCG93310.10390072564596387No Hit
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA92360.10284290023214258RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAAT92140.10259793013631027No Hit
GTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGA91990.10243090507097007No Hit
TACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAA90650.10093881448726423No Hit
GTAGAATTCTCGCCTGCCACGCGGGAGGCCCGGGTTTGGAATTCTCGGGT89980.1001927691954113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)